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| | <SX load='4atx' size='340' side='right' viewer='molstar' caption='[[4atx]], [[Resolution|resolution]] 8.20Å' scene=''> | | <SX load='4atx' size='340' side='right' viewer='molstar' caption='[[4atx]], [[Resolution|resolution]] 8.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4atx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4ATX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4atx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jff|1jff]], [[1sa0|1sa0]], [[1sa1|1sa1]], [[1tvk|1tvk]], [[1z2b|1z2b]], [[2wbe|2wbe]], [[2xrp|2xrp]], [[4aqv|4aqv]], [[4aqw|4aqw]], [[4atu|4atu]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tubulin_GTPase Tubulin GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.6 3.6.5.6] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atx OCA], [https://pdbe.org/4atx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atx RCSB], [https://www.ebi.ac.uk/pdbsum/4atx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4atx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atx OCA], [http://pdbe.org/4atx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4atx RCSB], [http://www.ebi.ac.uk/pdbsum/4atx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4atx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [[http://www.uniprot.org/uniprot/KINH_RAT KINH_RAT]] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).[UniProtKB:Q61768] [[http://www.uniprot.org/uniprot/TBA1D_BOVIN TBA1D_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). | + | [https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tubulin GTPase]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Fourniol, F J]] | + | [[Category: Fourniol FJ]] |
| - | [[Category: Fu, X]] | + | [[Category: Fu X]] |
| - | [[Category: Houdusse, A]] | + | [[Category: Houdusse A]] |
| - | [[Category: Jaiswal, J K]] | + | [[Category: Jaiswal JK]] |
| - | [[Category: Liu, J S]] | + | [[Category: Liu JS]] |
| - | [[Category: Moores, C A]] | + | [[Category: Moores CA]] |
| - | [[Category: Schubert, C R]] | + | [[Category: Schubert CR]] |
| - | [[Category: Stultz, C M]] | + | [[Category: Stultz CM]] |
| - | [[Category: Walsh, C A]] | + | [[Category: Walsh CA]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Microtubule]]
| + | |
| - | [[Category: Neck-linker]]
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| Structural highlights
Function
TBB2B_BOVIN Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Publication Abstract from PubMed
Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. Neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. Human disease-associated mutations in Dcx's linker sequence (e.g., W146C, K174E) alter Kif1a/Vamp2 transport by disrupting Dcx/Kif1a interactions without affecting Dcx MT binding. Dcx specifically enhances binding of the ADP-bound Kif1a motor domain to MTs. Cryo-electron microscopy and subnanometer-resolution image reconstruction reveal the kinesin-dependent conformational variability of MT-bound Dcx and suggest a model for MAP-motor crosstalk on MTs. Alteration of kinesin run length by MAPs represents a previously undiscovered mode of control of kinesin transport and provides a mechanism for regulation of MT-based transport by local signals.
Molecular basis for specific regulation of neuronal Kinesin-3 motors by doublecortin family proteins.,Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA, Walsh CA Mol Cell. 2012 Sep 14;47(5):707-21. doi: 10.1016/j.molcel.2012.06.025. Epub 2012 , Aug 1. PMID:22857951[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA, Walsh CA. Molecular basis for specific regulation of neuronal Kinesin-3 motors by doublecortin family proteins. Mol Cell. 2012 Sep 14;47(5):707-21. doi: 10.1016/j.molcel.2012.06.025. Epub 2012 , Aug 1. PMID:22857951 doi:http://dx.doi.org/10.1016/j.molcel.2012.06.025
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