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| | <SX load='4cy4' size='340' side='right' viewer='molstar' caption='[[4cy4]], [[Resolution|resolution]] 21.60Å' scene=''> | | <SX load='4cy4' size='340' side='right' viewer='molstar' caption='[[4cy4]], [[Resolution|resolution]] 21.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4cy4]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CY4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4CY4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4cy4]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CY4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4cy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cy4 OCA], [http://pdbe.org/4cy4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cy4 RCSB], [http://www.ebi.ac.uk/pdbsum/4cy4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cy4 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 21.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cy4 OCA], [https://pdbe.org/4cy4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cy4 RCSB], [https://www.ebi.ac.uk/pdbsum/4cy4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cy4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CORA_METJA CORA_METJA]] Mediates influx of magnesium ions.<ref>PMID:9573171</ref> | + | [https://www.uniprot.org/uniprot/CORA_METJA CORA_METJA] Mediates influx of magnesium ions.<ref>PMID:9573171</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Atcc 43067]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Baldock, C]] | + | [[Category: Methanocaldococcus jannaschii]] |
| - | [[Category: Cleverley, R M]] | + | [[Category: Baldock C]] |
| - | [[Category: Derrick, J P]] | + | [[Category: Cleverley RM]] |
| - | [[Category: Ford, R C]] | + | [[Category: Derrick JP]] |
| - | [[Category: Kean, J]] | + | [[Category: Ford RC]] |
| - | [[Category: Prince, S M]] | + | [[Category: Kean J]] |
| - | [[Category: Shintre, C A]] | + | [[Category: Prince SM]] |
| - | [[Category: Magnesium ion channel]]
| + | [[Category: Shintre CA]] |
| - | [[Category: Membrane protein]]
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| Structural highlights
Function
CORA_METJA Mediates influx of magnesium ions.[1]
Publication Abstract from PubMed
CorA channels are responsible for the uptake of essential magnesium ions by bacteria. X-ray crystal structures have been resolved for two full-length CorA channels, each in a non-conducting state with magnesium ions bound to the protein: These structures reveal a homo-pentameric quaternary structure with approximate 5-fold rotational symmetry about a central pore axis. We report the structure of the detergent solubilized Methanocaldococcus jannaschii CorA channel determined by Cryo-Electron Microscopy and Single Particle Averaging, supported by Small Angle X-ray Scattering and X-ray crystallography. This structure also shows a pentameric channel but with a highly asymmetric domain structure. The asymmetry of the domains includes differential separations between the trans-membrane segments, which reflects mechanical coupling of the cytoplasmic domain to the trans-membrane domain. This structure therefore reveals an important aspect of the gating mechanism of CorA channels by providing an indication of how the absence of magnesium ions leads to major structural changes.
The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions.,Cleverley RM, Kean J, Shintre CA, Baldock C, Derrick JP, Ford RC, Prince SM Biochim Biophys Acta. 2015 Jun 4. pii: S0005-2736(15)00180-7. doi:, 10.1016/j.bbamem.2015.06.002. PMID:26051127[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Smith RL, Gottlieb E, Kucharski LM, Maguire ME. Functional similarity between archaeal and bacterial CorA magnesium transporters. J Bacteriol. 1998 May;180(10):2788-91. PMID:9573171
- ↑ Cleverley RM, Kean J, Shintre CA, Baldock C, Derrick JP, Ford RC, Prince SM. The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions. Biochim Biophys Acta. 2015 Jun 4. pii: S0005-2736(15)00180-7. doi:, 10.1016/j.bbamem.2015.06.002. PMID:26051127 doi:http://dx.doi.org/10.1016/j.bbamem.2015.06.002
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