5uf6

<table><tr><td colspan='2'>[[5uf6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus Adeno-associated virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UF6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5UF6 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NTO:TRISULFOAMINO+HEPARIN+PENTASACCHARIDE'>NTO</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAPSID ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272636 Adeno-associated virus])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5uf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uf6 OCA], [http://pdbe.org/5uf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uf6 RCSB], [http://www.ebi.ac.uk/pdbsum/5uf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uf6 ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 A resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously derived by directed evolution, selecting for hepatocyte entry and resistance to neutralization by human serum. The structure of AAV-DJ differs from that of parental serotypes in two regions where neutralizing antibodies bind, so immune escape appears to have been the primary driver of AAV-DJ's directed evolution. Fondaparinux is an analog of cell surface heparan sulfate to which several AAVs bind during entry. Fondaparinux interacts with viral arginines at a known heparin binding site, without the large conformational changes whose presence was controversial in low-resolution imaging of AAV2-heparin complexes. The glycan density suggests multi-modal binding that could accommodate sequence variation and multivalent binding along a glycan polymer, consistent with a role in attachment, prior to more specific interactions with a receptor protein mediating entry.

 

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== References ==

[[Category: Chapman, M]]

[[Category: Davulcu, O]]

[[Category: Linhardt, R J]]

[[Category: Meyer, N L]]

[[Category: Noble, A J]]

[[Category: Sousa, D R]]

[[Category: Spear, J M]]

[[Category: Stagg, S M]]

[[Category: Xie, Q]]

[[Category: Zhang, F]]

[[Category: Virus like particle]]