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5wq7

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Current revision (10:16, 27 March 2024) (edit) (undo)
 
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<SX load='5wq7' size='340' side='right' viewer='molstar' caption='[[5wq7]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
<SX load='5wq7' size='340' side='right' viewer='molstar' caption='[[5wq7]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5wq7]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WQ7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5wq7]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WQ7 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wq8|5wq8]], [[5wq9|5wq9]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gspD, yheF, b3325, JW5707 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq7 OCA], [https://pdbe.org/5wq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wq7 RCSB], [https://www.ebi.ac.uk/pdbsum/5wq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq7 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq7 OCA], [http://pdbe.org/5wq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq7 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq7 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GSPD_ECOLI GSPD_ECOLI]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.
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[https://www.uniprot.org/uniprot/GSPD_ECOLI GSPD_ECOLI] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The secretin GspD of the type II secretion system (T2SS) forms a channel across the outer membrane in Gram-negative bacteria to transport substrates from the periplasm to the extracellular milieu. The lack of an atomic-resolution structure of the GspD channel hinders the investigation of substrate translocation mechanism of T2SS. Here we report cryo-EM structures of two GspD channels ( approximately 1 MDa), from Escherichia coli K12 and Vibrio cholerae, at approximately 3 A resolution. The structures reveal a pentadecameric channel architecture, wherein three rings of GspD N domains form the periplasmic channel. The secretin domain constitutes a novel double beta-barrel channel, with at least 60 beta-strands in each barrel, and is stabilized by S domains. The outer membrane channel is sealed by beta-strand-enriched gates. On the basis of the partially open state captured, we proposed a detailed gate-opening mechanism. Our structures provide a structural basis for understanding the secretin superfamily and the mechanism of substrate translocation in T2SS.
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Structural insights into the secretin translocation channel in the type II secretion system.,Yan Z, Yin M, Xu D, Zhu Y, Li X Nat Struct Mol Biol. 2017 Feb;24(2):177-183. doi: 10.1038/nsmb.3350. Epub 2017, Jan 9. PMID:28067918<ref>PMID:28067918</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5wq7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
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[[Category: Ecoli]]
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[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Li, X]]
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[[Category: Li X]]
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[[Category: Yan, Z]]
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[[Category: Yan Z]]
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[[Category: Yin, M]]
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[[Category: Yin M]]
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[[Category: C15 symmetry]]
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[[Category: Protein transport]]
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[[Category: Secretin family]]
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[[Category: T2ss]]
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Current revision

CryoEM structure of type II secretion system secretin GspD in E.coli K12

5wq7, resolution 3.04Å

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