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5y5x

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Current revision (10:21, 27 March 2024) (edit) (undo)
 
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<SX load='5y5x' size='340' side='right' viewer='molstar' caption='[[5y5x]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
<SX load='5y5x' size='340' side='right' viewer='molstar' caption='[[5y5x]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5y5x]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y5X FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5y5x]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y5X FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5x OCA], [http://pdbe.org/5y5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y5x RCSB], [http://www.ebi.ac.uk/pdbsum/5y5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5x ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5x OCA], [https://pdbe.org/5y5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y5x RCSB], [https://www.ebi.ac.uk/pdbsum/5y5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5x ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/VATE_THET8 VATE_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATD_THET8 VATD_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATC_THET8 VATC_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATB_THET8 VATB_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit. [[http://www.uniprot.org/uniprot/VATA_THET8 VATA_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit. [[http://www.uniprot.org/uniprot/VATF_THET8 VATF_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane.
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[https://www.uniprot.org/uniprot/VATA_THET8 VATA_THET8] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proton translocating rotary ATPases couple ATP hydrolysis/synthesis, which occurs in the soluble domain, with proton flow through the membrane domain via a rotation of the common central rotor complex against the surrounding peripheral stator apparatus. Here, we present a large data set of single particle cryo-electron micrograph images of the V/A type H(+)-rotary ATPase from the bacterium Thermus thermophilus, enabling the identification of three rotational states based on the orientation of the rotor subunit. Using masked refinement and classification with signal subtractions, we obtain homogeneous reconstructions for the whole complexes and soluble V1 domains. These reconstructions are of higher resolution than any EM map of intact rotary ATPase reported previously, providing a detailed molecular basis for how the rotary ATPase maintains structural integrity of the peripheral stator apparatus, and confirming the existence of a clear proton translocation path from both sides of the membrane.
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Cryo EM structure of intact rotary H(+)-ATPase/synthase from Thermus thermophilus.,Nakanishi A, Kishikawa JI, Tamakoshi M, Mitsuoka K, Yokoyama K Nat Commun. 2018 Jan 8;9(1):89. doi: 10.1038/s41467-017-02553-6. PMID:29311594<ref>PMID:29311594</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5y5x" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
*[[ATPase 3D structures|ATPase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thermus thermophilus hb8]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Kishikawa, J]]
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[[Category: Kishikawa J]]
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[[Category: Mitsuoka, K]]
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[[Category: Mitsuoka K]]
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[[Category: Nakanishi, A]]
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[[Category: Nakanishi A]]
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[[Category: Tamakoshi, M]]
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[[Category: Tamakoshi M]]
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[[Category: Yokoyama, K]]
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[[Category: Yokoyama K]]
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[[Category: Atp synthase]]
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[[Category: Bioenergetic]]
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[[Category: Motor protein]]
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[[Category: Proton pump]]
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[[Category: V-atpase]]
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Current revision

V/A-type ATPase/synthase from Thermus thermophilus, rotational state 1

5y5x, resolution 5.00Å

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