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| | <SX load='5z1f' size='340' side='right' viewer='molstar' caption='[[5z1f]], [[Resolution|resolution]] 4.80Å' scene=''> | | <SX load='5z1f' size='340' side='right' viewer='molstar' caption='[[5z1f]], [[Resolution|resolution]] 4.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5z1f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z1F FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z1f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z1F FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERD4, At1g30360, T4K22.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1f OCA], [http://pdbe.org/5z1f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z1f RCSB], [http://www.ebi.ac.uk/pdbsum/5z1f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1f ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1f OCA], [https://pdbe.org/5z1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z1f RCSB], [https://www.ebi.ac.uk/pdbsum/5z1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1f ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSCLD_ARATH CSCLD_ARATH]] Acts as an osmosensitive calcium-permeable cation channel. | + | [https://www.uniprot.org/uniprot/CSCLD_ARATH CSCLD_ARATH] Acts as an osmosensitive calcium-permeable cation channel. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Mechanosensitive ion channels convert mechanical stimuli into a flow of ions. These channels are widely distributed from bacteria to higher plants and humans, and are involved in many crucial physiological processes. Here we show that two members of the OSCA protein family in Arabidopsis thaliana, namely AtOSCA1.1 and AtOSCA3.1, belong to a new class of mechanosensitive ion channels. We solve the structure of the AtOSCA1.1 channel at 3.5-A resolution and AtOSCA3.1 at 4.8-A resolution by cryo-electron microscopy. OSCA channels are symmetric dimers that are mediated by cytosolic inter-subunit interactions. Strikingly, they have structural similarity to the mammalian TMEM16 family proteins. Our structural analysis accompanied with electrophysiological studies identifies the ion permeation pathway within each subunit and suggests a conformational change model for activation.
| + | |
| - | | + | |
| - | Structure of the mechanosensitive OSCA channels.,Zhang M, Wang D, Kang Y, Wu JX, Yao F, Pan C, Yan Z, Song C, Chen L Nat Struct Mol Biol. 2018 Sep;25(9):850-858. doi: 10.1038/s41594-018-0117-6. Epub, 2018 Sep 6. PMID:30190597<ref>PMID:30190597</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5z1f" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, L]] | + | [[Category: Chen L]] |
| - | [[Category: Kang, Y]] | + | [[Category: Kang Y]] |
| - | [[Category: Wu, J X]] | + | [[Category: Wu JX]] |
| - | [[Category: Zhang, M]] | + | [[Category: Zhang M]] |
| - | [[Category: Ion channel]]
| + | |
| - | [[Category: Mechanosensitive]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Metal transport]]
| + | |
| - | [[Category: Osca]]
| + | |
| - | [[Category: Osmosensing]]
| + | |
| - | [[Category: Tmem63]]
| + | |
