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| | <SX load='5zx5' size='340' side='right' viewer='molstar' caption='[[5zx5]], [[Resolution|resolution]] 3.28Å' scene=''> | | <SX load='5zx5' size='340' side='right' viewer='molstar' caption='[[5zx5]], [[Resolution|resolution]] 3.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zx5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZX5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZX5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zx5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZX5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.28Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trpm7, Chak, Ltrpc7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zx5 OCA], [https://pdbe.org/5zx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zx5 RCSB], [https://www.ebi.ac.uk/pdbsum/5zx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zx5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zx5 OCA], [http://pdbe.org/5zx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zx5 RCSB], [http://www.ebi.ac.uk/pdbsum/5zx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zx5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TRPM7_MOUSE TRPM7_MOUSE]] Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).<ref>PMID:11385574</ref> | + | [https://www.uniprot.org/uniprot/TRPM7_MOUSE TRPM7_MOUSE] Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).<ref>PMID:11385574</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Non-specific serine/threonine protein kinase]]
| + | [[Category: Abiria SA]] |
| - | [[Category: Abiria, S A]] | + | [[Category: Clapham DE]] |
| - | [[Category: Clapham, D E]] | + | [[Category: Duan J]] |
| - | [[Category: Duan, J]] | + | [[Category: Hulse RE]] |
| - | [[Category: Hulse, R E]] | + | [[Category: Krapivinsky G]] |
| - | [[Category: Krapivinsky, G]] | + | [[Category: Li J]] |
| - | [[Category: Li, J]] | + | [[Category: Li Z]] |
| - | [[Category: Li, Z]] | + | [[Category: Santa-Cruz A]] |
| - | [[Category: Santa-Cruz, A]] | + | [[Category: Zhang J]] |
| - | [[Category: Zhang, J]] | + | |
| - | [[Category: Cryoem]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Truncated mouse trpm7]]
| + | |
| Structural highlights
Function
TRPM7_MOUSE Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).[1]
Publication Abstract from PubMed
The transient receptor potential ion channel subfamily M, member 7 (TRPM7), is a ubiquitously expressed protein that is required for mouse embryonic development. TRPM7 contains both an ion channel and an alpha-kinase. The channel domain comprises a nonselective cation channel with notable permeability to Mg(2+) and Zn(2+) Here, we report the closed state structures of the mouse TRPM7 channel domain in three different ionic conditions to overall resolutions of 3.3, 3.7, and 4.1 A. The structures reveal key residues for an ion binding site in the selectivity filter, with proposed partially hydrated Mg(2+) ions occupying the center of the conduction pore. In high [Mg(2+)], a prominent external disulfide bond is found in the pore helix, which is essential for ion channel function. Our results provide a structural framework for understanding the TRPM1/3/6/7 subfamily and extend the knowledge base upon which to study the diversity and evolution of TRP channels.
Structure of the mammalian TRPM7, a magnesium channel required during embryonic development.,Duan J, Li Z, Li J, Hulse RE, Santa-Cruz A, Valinsky WC, Abiria SA, Krapivinsky G, Zhang J, Clapham DE Proc Natl Acad Sci U S A. 2018 Aug 14. pii: 1810719115. doi:, 10.1073/pnas.1810719115. PMID:30108148[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nadler MJ, Hermosura MC, Inabe K, Perraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet JP, Penner R, Scharenberg AM, Fleig A. LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability. Nature. 2001 May 31;411(6837):590-5. PMID:11385574 doi:http://dx.doi.org/10.1038/35079092
- ↑ Duan J, Li Z, Li J, Hulse RE, Santa-Cruz A, Valinsky WC, Abiria SA, Krapivinsky G, Zhang J, Clapham DE. Structure of the mammalian TRPM7, a magnesium channel required during embryonic development. Proc Natl Acad Sci U S A. 2018 Aug 14. pii: 1810719115. doi:, 10.1073/pnas.1810719115. PMID:30108148 doi:http://dx.doi.org/10.1073/pnas.1810719115
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