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| | <SX load='6izv' size='340' side='right' viewer='molstar' caption='[[6izv]], [[Resolution|resolution]] 4.20Å' scene=''> | | <SX load='6izv' size='340' side='right' viewer='molstar' caption='[[6izv]], [[Resolution|resolution]] 4.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6izv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_prevot_594 Clostridium botulinum prevot_594]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6IZV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6izv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_Prevot_594 Clostridium botulinum Prevot_594]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IZV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ixw|6ixw]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">T258_3831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1408284 Clostridium botulinum Prevot_594])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6izv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izv OCA], [https://pdbe.org/6izv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6izv RCSB], [https://www.ebi.ac.uk/pdbsum/6izv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6izv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6izv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6izv OCA], [http://pdbe.org/6izv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6izv RCSB], [http://www.ebi.ac.uk/pdbsum/6izv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6izv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Clostridium botulinum prevot_594]] | + | [[Category: Clostridium botulinum Prevot_594]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dandey, V P]] | + | [[Category: Dandey VP]] |
| - | [[Category: Koh, F]] | + | [[Category: Koh F]] |
| - | [[Category: Lee, L J]] | + | [[Category: Lee LJ]] |
| - | [[Category: Narita, A]] | + | [[Category: Narita A]] |
| - | [[Category: Popp, D]] | + | [[Category: Popp D]] |
| - | [[Category: Robinson, R C]] | + | [[Category: Robinson RC]] |
| - | [[Category: Tan, Y Z]] | + | [[Category: Tan YZ]] |
| - | [[Category: Tanaka, K]] | + | [[Category: Tanaka K]] |
| - | [[Category: Cytoskeleton]]
| + | |
| - | [[Category: Filament]]
| + | |
| - | [[Category: Parm]]
| + | |
| - | [[Category: Protein fibril]]
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| Structural highlights
Publication Abstract from PubMed
Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.
The structure of a 15-stranded actin-like filament from Clostridium botulinum.,Koh F, Narita A, Lee LJ, Tanaka K, Tan YZ, Dandey VP, Popp D, Robinson RC Nat Commun. 2019 Jun 28;10(1):2856. doi: 10.1038/s41467-019-10779-9. PMID:31253774[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Koh F, Narita A, Lee LJ, Tanaka K, Tan YZ, Dandey VP, Popp D, Robinson RC. The structure of a 15-stranded actin-like filament from Clostridium botulinum. Nat Commun. 2019 Jun 28;10(1):2856. doi: 10.1038/s41467-019-10779-9. PMID:31253774 doi:http://dx.doi.org/10.1038/s41467-019-10779-9
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