6j6j

From Proteopedia

(Difference between revisions)

Current revision

Biotin-bound streptavidin

6j6j, resolution 3.20Å

Structural highlights

6j6j is a 4 chain structure with sequence from Streptomyces avidinii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.2Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAV_STRAV The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Publication Abstract from PubMed

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 A resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.,Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW Nat Commun. 2019 Jun 3;10(1):2386. doi: 10.1038/s41467-019-10368-w. PMID:31160591^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW. Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat Commun. 2019 Jun 3;10(1):2386. doi: 10.1038/s41467-019-10368-w. PMID:31160591 doi:http://dx.doi.org/10.1038/s41467-019-10368-w

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6j6j"

@@ Line 3: / Line 3: @@
 <SX load='6j6j' size='340' side='right' viewer='molstar' caption='[[6j6j]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6j6j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J6J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6j6j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J6J FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6j OCA], [http://pdbe.org/6j6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j6j RCSB], [http://www.ebi.ac.uk/pdbsum/6j6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6j ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6j OCA], [https://pdbe.org/6j6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j6j RCSB], [https://www.ebi.ac.uk/pdbsum/6j6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6j ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
+[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 6j6j" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Avidin 3D structures|Avidin 3D structures]]
 == References ==
 <references/>
@@ Line 24: / Line 28: @@
 [[Category: Large Structures]]
 [[Category: Streptomyces avidinii]]
-[[Category: Fan, X]]
+[[Category: Fan X]]
-[[Category: Lei, J L]]
+[[Category: Lei JL]]
-[[Category: Wang, H W]]
+[[Category: Wang HW]]
-[[Category: Wang, J]]
+[[Category: Wang J]]
-[[Category: Cytosolic protein]]
-[[Category: Streptavidin]]

6j6j

From Proteopedia

Current revision

Biotin-bound streptavidin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox