6n1i

From Proteopedia

(Difference between revisions)

Current revision

Cryo-EM structure of NLRC4-CARD filament

6n1i, resolution 3.58Å

Structural highlights

6n1i is a 16 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.58Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

NLRC4_HUMAN The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.

Function

NLRC4_HUMAN Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]^[1]

Publication Abstract from PubMed

Canonical inflammasomes are cytosolic supramolecular complexes that activate caspase-1 upon sensing extrinsic microbial invasions and intrinsic sterile stress signals. During inflammasome assembly, adaptor proteins ASC and NLRC4 recruit caspase-1 through homotypic caspase recruitment domain (CARD) interactions, leading to caspase-1 dimerization and activation. Activated caspase-1 processes proinflammatory cytokines and Gasdermin D to induce cytokine maturation and pyroptotic cell death. Here, we present cryo-electron microscopy (cryo-EM) structures of NLRC4 CARD and ASC CARD filaments mediated by conserved three types of asymmetric interactions (types I, II, and III). We find that the CARDs of these two adaptor proteins share a similar assembly pattern, which matches that of the caspase-1 CARD filament whose structure we defined previously. These data indicate a unified mechanism for downstream caspase-1 recruitment through CARD-CARD interactions by both adaptors. Using structure modeling, we further show that full-length NLRC4 assembles via two separate symmetries at its CARD and its nucleotide-binding domain (NBD), respectively.

Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1.,Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H Proc Natl Acad Sci U S A. 2018 Oct 2. pii: 1810524115. doi:, 10.1073/pnas.1810524115. PMID:30279182^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Damiano JS, Oliveira V, Welsh K, Reed JC. Heterotypic interactions among NACHT domains: implications for regulation of innate immune responses. Biochem J. 2004 Jul 1;381(Pt 1):213-9. doi: 10.1042/BJ20031506. PMID:15107016 doi:http://dx.doi.org/10.1042/BJ20031506
↑ Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H. Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1. Proc Natl Acad Sci U S A. 2018 Oct 2. pii: 1810524115. doi:, 10.1073/pnas.1810524115. PMID:30279182 doi:http://dx.doi.org/10.1073/pnas.1810524115

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6n1i"

Categories: Homo sapiens | Large Structures | Fu T | Li Y | Wu H

@@ Line 3: / Line 3: @@
 <SX load='6n1i' size='340' side='right' viewer='molstar' caption='[[6n1i]], [[Resolution|resolution]] 3.58&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6drp 6drp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6n1i]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N1I FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [http://pdbe.org/6n1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [http://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.58&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n1i OCA], [https://pdbe.org/6n1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n1i RCSB], [https://www.ebi.ac.uk/pdbsum/6n1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n1i ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.
+[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] The disease is caused by mutations affecting the gene represented in this entry.  The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN]] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
+[https://www.uniprot.org/uniprot/NLRC4_HUMAN NLRC4_HUMAN] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]<ref>PMID:15107016</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 __TOC__
 </SX>
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Fu, T]]
+[[Category: Fu T]]
-[[Category: Li, Y]]
+[[Category: Li Y]]
-[[Category: Wu, H]]
+[[Category: Wu H]]
-[[Category: Filament]]
-[[Category: Immunity]]
-[[Category: Inflammasome]]
-[[Category: Signaling protein]]

6n1i

From Proteopedia

Current revision

Cryo-EM structure of NLRC4-CARD filament

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox