|
|
| Line 3: |
Line 3: |
| | <SX load='6oua' size='340' side='right' viewer='molstar' caption='[[6oua]], [[Resolution|resolution]] 4.18Å' scene=''> | | <SX load='6oua' size='340' side='right' viewer='molstar' caption='[[6oua]], [[Resolution|resolution]] 4.18Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6oua]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OUA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6oua]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OUA FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6nuw|6nuw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.18Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oua OCA], [http://pdbe.org/6oua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oua RCSB], [http://www.ebi.ac.uk/pdbsum/6oua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oua ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oua OCA], [https://pdbe.org/6oua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oua RCSB], [https://www.ebi.ac.uk/pdbsum/6oua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oua ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CENPI_YEAST CENPI_YEAST]] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:12408861</ref> [[http://www.uniprot.org/uniprot/CENPK_YEAST CENPK_YEAST]] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:9987135</ref> [[http://www.uniprot.org/uniprot/CENPH_YEAST CENPH_YEAST]] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:9862478</ref> | + | [https://www.uniprot.org/uniprot/CENPI_YEAST CENPI_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:12408861</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.
| + | |
| | | | |
| - | The structure of the yeast Ctf3 complex.,Hinshaw SM, Dates AN, Harrison SC Elife. 2019 Jun 13;8. pii: 48215. doi: 10.7554/eLife.48215. PMID:31194673<ref>PMID:31194673</ref>
| + | ==See Also== |
| - | | + | *[[Centromere protein 3D structure|Centromere protein 3D structure]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6oua" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Hinshaw, S M]] | + | [[Category: Harrison SC]] |
| - | [[Category: Cell cycle]] | + | [[Category: Hinshaw SM]] |
| - | [[Category: Chromosome]]
| + | |
| - | [[Category: Cryo-em]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
