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Tailspike protein

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<StructureSection load='3th0' size='340' side='right' caption='Glycosylated tailspike protein from bacteriophage P222 complex with octasaccharide (PDB code [[3th0]])' scene=''>
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<StructureSection load='3th0' size='340' side='right' caption='Glycosylated tailspike protein from bacteriophage P222 complex with octasaccharide (PDB code [[3th0]])' scene='84/841073/Cv/1'>
== Function ==
== Function ==
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'''Tailspike protein''' (TSP) of bacteriophage P22 is a trimeric protein which serves as the receptor-binding protein of the bacteriophage to its bacterial host<ref>PMID:29500571</ref>. O-antigen polysaccharides found on the outer surface of bacteria are the natural substrates for TSP<ref>PMID:20817910</ref>.
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The '''tailspike protein''' (TSP) of bacteriophage is a trimeric protein which serves as the receptor-binding protein of the bacteriophage to its bacterial host<ref>PMID:29500571</ref>. O-antigen polysaccharides found on the outer surface of bacteria are the natural substrates for TSP<ref>PMID:20817910</ref>.
== Relevance ==
== Relevance ==
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Bacteriophage research is becoming important due to antibiotics resistance. TSP binding to pathogenic bacteria can be used for their detection<ref>PMID:30111705</ref>.
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Bacteriophage research is becoming important due to the problem of antibiotics resistance. TSP binding to pathogenic bacteria can be used for their detection<ref>PMID:30111705</ref>.
== Structural highlights ==
== Structural highlights ==
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The C-terminal domain (CTD) (residues 109-end) of TSP is responsible for the attachment of the tailspike protein to the O-antigen moiety on bacterial cell surface. The 3D structure of an octasaccharide bound to CTP of TSP shows numerous hydrophobic interactions as well as well defined water molecules forming hydrogen bonds to the antigen<ref>PMID:30111705</ref>.
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The C-terminal domain (CTD) (residues 109-end) of TSP is responsible for the attachment of the tailspike protein to the O-antigen moiety on bacterial cell surface. The 3D structure of an <scene name='84/841073/Cv/3'>octasaccharide</scene> bound to CTP of TSP shows <scene name='84/841073/Cv/7'>numerous hydrophobic interactions as well as defined water molecules forming hydrogen bonds to the antigen</scene><ref>PMID:23292517</ref>.
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</StructureSection>
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==3D structures of tailspike protein==
==3D structures of tailspike protein==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[Tailspike protein 3D structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Full size tailspike protein
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**[[2xc1]] - P22TSP - bacteriophage P22<br />
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**[[5w6p]] - CBA120TSP2 - bacteriophage CBA120<br />
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**[[6nw9]], [[5w6f]] - CBA120TSP3<br />
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**[[5js4]] - AB6TSP - bacteriophage PHIAB6<br />
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**[[5jsd]] - AB6TSP + polysaccharide<br />
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**[[4ru5]] - TSP - bacteriophage PHI297<br />
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**[[5jse]] - TSP + polysaccharide - bacteriophage PHIAB6<br />
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**[[4qnl]] - TSP - bacteriophage G7C<br />
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*Tailspike protein N-terminal NTD 1-108
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**[[1lkt]] - P22TSP NTD<br />
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**[[2vky]] - P22TSP NTD/isoleucine zipper<br />
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**[[2vnl]] - P22TSP NTD/isoleucine zipper (mutant)<br />
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**[[2vji]] - HK620TSP NTD - bacteriophage HK620<br />
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*Tailspike protein C-terminal CTD 109-end
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**[[1tyv]], [[1tyu]], [[1tyx]], [[1tyw]], [[2vfm]], [[1tsp]] - P22TSP CTD <br />
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**[[1qq1]], [[1qrc]], [[1qrb]], [[2vfp]], [[2vfo]], [[2vfq]], [[1qa3]], [[1qa2]], [[1qa1]], [[2vfn]], [[1clw]] - P22TSP CTD (mutant)<br />
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**[[4avz]], [[4xon]], [[4xqf]], [[4xlc]], [[4xl9]], [[4xkv]], [[4xlh]], [[4xnf]], [[4xn0]] - HK520TSP CTD (mutant)<br />
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**[[2vbe]] - TSP CTD - bacteriophage SFVI<br />
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**[[2vbk]] - SF6TSP CTD - bacteriophage SF6<br />
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**[[5w6h]] - CBA120TSP4 active domain 344-1056<br />
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**[[6f7d]], [[6f7k]], [[2v5i]] - SF6TSP CTD - bacteriophage DET7<br />>
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**[[6e0v]] - PHI92TSP CTD - bacteriophage PHI92<br />
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**[[6e1r]] - TSP CTD - bacteriophage VB_APIP_P1<br />
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**[[6eu4]] - TSP CTD - bacteriophage VB_ABAP_AS12<br />
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**[[5w5p]] - TSP CTD - bacteriophage AM24<br />
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**[[4ru4]] - TSP CTD - bacteriophage LKA1<br />
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**[[3gvl]] - KIFTSP CTD - bacteriophage KIF<br />
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**[[3gvj]], [[3gvk]] - KIFTSP CTD (mutant)<br />
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**[[3riq]] - TSP CTD - siphovirus 9NA<br />
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*Tailspike protein CTD complex with polysaccharides
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**[[3th0]] - P22TSP CTD + octasaccharide<br />
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**[[2x85]], [[2vjj]], [[2x6x]], [[6g0x]] - HK520TSP CTD + polysaccharide<br />
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**[[2x6w]], [[2x6y]], [[4xla]], [[5xop]], [[5xor]], [[4xr6]], [[4yej]], [[4yel]], [[4xle]], [[4xlf]], [[4xkw]], [[4xm3]], [[4xmy]], [[4xn3]], [[4xot]], [[6gvp]], [[6gvr]] - HK520TSP CTD (mutant) + polysaccharide<br />
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**[[2vbm]], [[4urr]] - SF6TSP CTD + polysaccharide<br />
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**[[5w6s]] - CBA120TSP4 active domain + polysaccharide<br />
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**[[5w6s]] - CBA120TSP4 active domain + polysaccharide<br />
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**[[6e0w]] - PHI92TSP CTD + colanic acid<br />
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}}
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Glycosylated tailspike protein from bacteriophage P222 complex with octasaccharide (PDB code 3th0)

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Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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