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| | <StructureSection load='2j1o' size='340' side='right'caption='[[2j1o]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='2j1o' size='340' side='right'caption='[[2j1o]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2j1o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brassica_hirta Brassica hirta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2J1O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2j1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J1O FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j1p|2j1p]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1o OCA], [http://pdbe.org/2j1o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1o RCSB], [http://www.ebi.ac.uk/pdbsum/2j1o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1o ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1o OCA], [https://pdbe.org/2j1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j1o RCSB], [https://www.ebi.ac.uk/pdbsum/2j1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1o ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GGPPS_SINAL GGPPS_SINAL] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Brassica hirta]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Beyer, P]] | + | [[Category: Sinapis alba]] |
| - | [[Category: Kloer, D P]] | + | [[Category: Beyer P]] |
| - | [[Category: Schulz, G E]] | + | [[Category: Kloer DP]] |
| - | [[Category: Welsch, R]] | + | [[Category: Schulz GE]] |
| - | [[Category: Carotenoid biosynthesis]] | + | [[Category: Welsch R]] |
| - | [[Category: Chloroplast]]
| + | |
| - | [[Category: Isoprene biosynthesis]]
| + | |
| - | [[Category: Isoprenoid diphosphate synthase]]
| + | |
| - | [[Category: Isoprenyl transtransferase]]
| + | |
| - | [[Category: Multifunctional enzyme]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transit peptide]]
| + | |
| Structural highlights
Function
GGPPS_SINAL
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of the geranylgeranyl diphosphate synthase from Sinapis alba (mustard) has been solved in two crystal forms at 1.8 and 2.0 A resolutions. In one of these forms, the dimeric enzyme binds one molecule of the final product geranylgeranyl diphosphate in one subunit. The chainfold of the enzyme corresponds to that of other members of the farnesyl diphosphate synthase family. Whereas the binding modes of the two substrates dimethylallyl diphosphate and isopentenyl diphosphate at the allyl and isopentenyl sites, respectively, have been established with other members of the family, the complex structure presented reveals for the first time the binding mode of a reaction product at the isopentenyl site. The binding geometry of substrates and product in conjunction with the protein environment and the established chemistry of the reaction provide a clear picture of the reaction steps and atom displacements. Moreover, a comparison with a ligated homologous structure outlined an appreciable induced fit: helix alpha8 and its environment undergo a large conformational change when either the substrate dimethylallyl diphosphate or an analogue is bound to the allyl site; only a minor conformational change occurs when the other substrate isopentenyl diphosphate or the product is bound to the isopentenyl site.
Structure and reaction geometry of geranylgeranyl diphosphate synthase from Sinapis alba.,Kloer DP, Welsch R, Beyer P, Schulz GE Biochemistry. 2006 Dec 26;45(51):15197-204. Epub 2006 Dec 1. PMID:17176041[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kloer DP, Welsch R, Beyer P, Schulz GE. Structure and reaction geometry of geranylgeranyl diphosphate synthase from Sinapis alba. Biochemistry. 2006 Dec 26;45(51):15197-204. Epub 2006 Dec 1. PMID:17176041 doi:10.1021/bi061572k
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