6wim
From Proteopedia
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(New page: '''Unreleased structure''' The entry 6wim is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==CdiB from Escherichia coli== | |
| + | <StructureSection load='6wim' size='340' side='right'caption='[[6wim]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WIM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wim OCA], [https://pdbe.org/6wim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wim RCSB], [https://www.ebi.ac.uk/pdbsum/6wim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wim ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial contact-dependent growth inhibition (CDI) systems use a type Vb secretion mechanism to export large CdiA toxins across the outer membrane by dedicated outer membrane transporters called CdiB. Here we report the first crystal structures of two CdiB transporters from Acinetobacter baumannii and Escherichia coli. CdiB transporters adopt a TpsB fold, containing a 16-stranded transmembrane beta-barrel connected to two periplasmic domains. The lumen of the CdiB pore is occluded by an N-terminal alpha-helix and the conserved extracellular loop 6; these two elements adopt different conformations in the structures. We identified a conserved DxxG motif located on strand beta1 that connects loop 6 through different networks of interactions. Structural modifications of DxxG induce rearrangement of extracellular loops and alter interactions with the N-terminal alpha-helix, preparing the system for alpha-helix ejection. Using structural biology, functional assays, and molecular dynamics simulations, we show how the barrel pore is primed for CdiA toxin secretion. | ||
| - | + | Structural insight into toxin secretion by contact dependent growth inhibition transporters.,Guerin J, Botos I, Zhang Z, Lundquist K, Gumbart JC, Buchanan SK Elife. 2020 Oct 22;9. pii: 58100. doi: 10.7554/eLife.58100. PMID:33089781<ref>PMID:33089781</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6wim" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Botos I]] | ||
| + | [[Category: Buchanan SK]] | ||
| + | [[Category: Guerin J]] | ||
Current revision
CdiB from Escherichia coli
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