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6uwl
From Proteopedia
(Difference between revisions)
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==GltPh in complex with L-aspartate and sodium ions in intermediate outward-facing state== | ==GltPh in complex with L-aspartate and sodium ions in intermediate outward-facing state== | ||
| - | <StructureSection load='6uwl' size='340' side='right'caption='[[6uwl]]' scene=''> | + | <StructureSection load='6uwl' size='340' side='right'caption='[[6uwl]], [[Resolution|resolution]] 3.62Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UWL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UWL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6uwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UWL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UWL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uwl OCA], [http://pdbe.org/6uwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uwl RCSB], [http://www.ebi.ac.uk/pdbsum/6uwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uwl ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uwl OCA], [http://pdbe.org/6uwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uwl RCSB], [http://www.ebi.ac.uk/pdbsum/6uwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uwl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In proteins where conformational changes are functionally important, the number of accessible states and their dynamics are often difficult to establish. Here we describe a novel (19)F-NMR spectroscopy approach to probe dynamics of large membrane proteins. We labeled a glutamate transporter homolog with a (19)F probe via cysteine chemistry and with a Ni(2+) ion via chelation by a di-histidine motif. We used distance-dependent enhancement of the longitudinal relaxation of (19)F nuclei by the paramagnetic metal to assign the observed resonances. We identified one inward- and two outward-facing states of the transporter, in which the substrate-binding site is near the extracellular and intracellular solutions, respectively. We then resolved the structure of the unanticipated second outward-facing state by cryo-EM. Finally, we showed that the rates of the conformational exchange are accessible from measurements of the metal-enhanced longitudinal relaxation of (19)F nuclei. | ||
| + | |||
| + | Use of paramagnetic (19)F NMR to monitor domain movement in a glutamate transporter homolog.,Huang Y, Wang X, Lv G, Razavi AM, Huysmans GHM, Weinstein H, Bracken C, Eliezer D, Boudker O Nat Chem Biol. 2020 Sep;16(9):1006-1012. doi: 10.1038/s41589-020-0561-6. Epub, 2020 Jun 8. PMID:32514183<ref>PMID:32514183</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6uwl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Pyrococcus shinkaii]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Boudker O]] | + | [[Category: Boudker, O]] |
| - | [[Category: Wang X]] | + | [[Category: Wang, X]] |
| + | [[Category: Aspartate transporter]] | ||
| + | [[Category: Transport protein]] | ||
Current revision
GltPh in complex with L-aspartate and sodium ions in intermediate outward-facing state
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