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2ker

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alpha-amylase inhibitor Parvulustat (Z-2685) from Streptomyces parvulus

Structural highlights

2ker is a 1 chain structure with sequence from Atcc 12434. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IAA_STRRO] Inhibits mammalian alpha-amylases specifically but has no action on plant and microbial alpha-amylases.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The protein parvulustat (Z-2685) from Streptomyces parvulus comprises 78 amino acids and functions as a highly efficient alpha-amylase inhibitor. Parvulustat shares 29.6 % overall amino acid sequence identity to the well-known alpha-amylase inhibitor tendamistat. Among the conserved residues are the two disulfide bridges (C9-C25, C43-C70) and the active-site motif (W16, R17, Y18). Here, we report the high-resolution NMR structure of parvulustat based on NOEs, J couplings, chemical shifts and hydrogen-exchange data. In addition, we studied the dynamical properties of parvulustat by heteronuclear relaxation measurements. We compare the structure of parvulustat with the structure of tendamistat in terms of secondary structure elements, charges and hydrophobicity. The overall structural composition is very similar, but there are distinct differences including the active-site region. These structural and dynamical differences indicate that for parvulustat an induced-fit mechanism for binding to alpha-amylase might take place, since the structure of tendamistat does not change upon binding to alpha-amylase.

The high resolution NMR structure of parvulustat (Z-2685) from Streptomyces parvulus FH-1641: comparison with tendamistat from Streptomyces tendae 4158.,Rehm S, Han S, Hassani I, Sokocevic A, Jonker HR, Engels JW, Schwalbe H Chembiochem. 2009 Jan 5;10(1):119-27. PMID:19067455^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rehm S, Han S, Hassani I, Sokocevic A, Jonker HR, Engels JW, Schwalbe H. The high resolution NMR structure of parvulustat (Z-2685) from Streptomyces parvulus FH-1641: comparison with tendamistat from Streptomyces tendae 4158. Chembiochem. 2009 Jan 5;10(1):119-27. PMID:19067455 doi:10.1002/cbic.200800547

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ker"

@@ Line 3: / Line 3: @@
 <StructureSection load='2ker' size='340' side='right'caption='[[2ker]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ker]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12434 Atcc 12434]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KER OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2KER FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ker]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_12434 Atcc 12434]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KER FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ker OCA], [http://pdbe.org/2ker PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ker RCSB], [http://www.ebi.ac.uk/pdbsum/2ker PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ker ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ker FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ker OCA], [https://pdbe.org/2ker PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ker RCSB], [https://www.ebi.ac.uk/pdbsum/2ker PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ker ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IAA_STRRO IAA_STRRO]] Inhibits mammalian alpha-amylases specifically but has no action on plant and microbial alpha-amylases.
+[[https://www.uniprot.org/uniprot/IAA_STRRO IAA_STRRO]] Inhibits mammalian alpha-amylases specifically but has no action on plant and microbial alpha-amylases.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2ker

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Current revision

alpha-amylase inhibitor Parvulustat (Z-2685) from Streptomyces parvulus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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