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| | <StructureSection load='5ed8' size='340' side='right'caption='[[5ed8]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='5ed8' size='340' side='right'caption='[[5ed8]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ed8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ED8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ed8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ED8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ED8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mKIAA0668 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ed8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed8 OCA], [http://pdbe.org/5ed8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ed8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ed8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ed8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ed8 OCA], [https://pdbe.org/5ed8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ed8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ed8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ed8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/SUN2_MOUSE SUN2_MOUSE] Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Specifically, Syne2 and Sun2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.<ref>PMID:16380439</ref> <ref>PMID:19509342</ref> <ref>PMID:19843581</ref> <ref>PMID:19874786</ref> <ref>PMID:20724637</ref> |
| - | SUN proteins are the core components of LINC complexes that span across the nuclear envelope for nuclear positioning and migration. SUN proteins contain at least one predicted coiled-coil domain preceding the SUN domain. Here, we found that the two coiled-coil domains (CC1 and CC2) of SUN2 exhibit distinct oligomeric states. CC2 is a monomer in solution. The structure of the CC2-SUN monomer revealed that CC2 unexpectedly folds as a three-helix bundle that interacts with the SUN domain and locks it in an inactive conformation. In contrast, CC1 is a trimer. The structure of the CC1 trimer demonstrated that CC1 is an imperfect coiled coil for the trimerization and activation of the SUN domain. Modulations of CC1 and CC2 dictate different oligomeric states of CC1-CC2-SUN, which is essential for LINC complex formation. Thus, the two coiled-coil domains of SUN2 act as the intrinsic dynamic regulators for controlling the SUN domain activity. | + | |
| - | | + | |
| - | Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators.,Nie S, Ke H, Gao F, Ren J, Wang M, Huo L, Gong W, Feng W Structure. 2016 Jan 5;24(1):80-91. doi: 10.1016/j.str.2015.10.024. Epub 2015 Dec , 10. PMID:26688217<ref>PMID:26688217</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| - | <div class="pdbe-citations 5ed8" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Feng, W]] | + | [[Category: Feng W]] |
| - | [[Category: Gao, F]] | + | [[Category: Gao F]] |
| - | [[Category: Gong, W M]] | + | [[Category: Gong WM]] |
| - | [[Category: Huo, L]] | + | [[Category: Huo L]] |
| - | [[Category: Ke, H M]] | + | [[Category: Ke HM]] |
| - | [[Category: Nie, S]] | + | [[Category: Nie S]] |
| - | [[Category: Ren, J Q]] | + | [[Category: Ren JQ]] |
| - | [[Category: Wang, M Z]] | + | [[Category: Wang MZ]] |
| - | [[Category: Nuclear membrane]]
| + | |
| - | [[Category: Sun domain]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
SUN2_MOUSE Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Specifically, Syne2 and Sun2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.[1] [2] [3] [4] [5]
References
- ↑ Crisp M, Liu Q, Roux K, Rattner JB, Shanahan C, Burke B, Stahl PD, Hodzic D. Coupling of the nucleus and cytoplasm: role of the LINC complex. J Cell Biol. 2006 Jan 2;172(1):41-53. Epub 2005 Dec 27. PMID:16380439 doi:http://dx.doi.org/10.1083/jcb.200509124
- ↑ Lei K, Zhang X, Ding X, Guo X, Chen M, Zhu B, Xu T, Zhuang Y, Xu R, Han M. SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice. Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10207-12. doi:, 10.1073/pnas.0812037106. Epub 2009 Jun 9. PMID:19509342 doi:http://dx.doi.org/10.1073/pnas.0812037106
- ↑ Ostlund C, Folker ES, Choi JC, Gomes ER, Gundersen GG, Worman HJ. Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteins. J Cell Sci. 2009 Nov 15;122(Pt 22):4099-108. doi: 10.1242/jcs.057075. Epub 2009, Oct 20. PMID:19843581 doi:http://dx.doi.org/10.1242/jcs.057075
- ↑ Zhang X, Lei K, Yuan X, Wu X, Zhuang Y, Xu T, Xu R, Han M. SUN1/2 and Syne/Nesprin-1/2 complexes connect centrosome to the nucleus during neurogenesis and neuronal migration in mice. Neuron. 2009 Oct 29;64(2):173-87. doi: 10.1016/j.neuron.2009.08.018. PMID:19874786 doi:http://dx.doi.org/10.1016/j.neuron.2009.08.018
- ↑ Luxton GW, Gomes ER, Folker ES, Vintinner E, Gundersen GG. Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement. Science. 2010 Aug 20;329(5994):956-9. doi: 10.1126/science.1189072. PMID:20724637 doi:http://dx.doi.org/10.1126/science.1189072
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