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| | <StructureSection load='5eog' size='340' side='right'caption='[[5eog]], [[Resolution|resolution]] 3.05Å' scene=''> | | <StructureSection load='5eog' size='340' side='right'caption='[[5eog]], [[Resolution|resolution]] 3.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5eog]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EOG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5eog]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EOG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.05Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MAB21L1, CAGR1, Nbla00126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5eog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eog OCA], [http://pdbe.org/5eog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eog RCSB], [http://www.ebi.ac.uk/pdbsum/5eog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eog ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eog OCA], [https://pdbe.org/5eog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eog RCSB], [https://www.ebi.ac.uk/pdbsum/5eog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eog ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MB211_HUMAN MB211_HUMAN]] Required for several aspects of embryonic development including normal development of the eye. | + | [https://www.uniprot.org/uniprot/MB211_HUMAN MB211_HUMAN] Required for several aspects of embryonic development including normal development of the eye. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
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| - | The exceptionally conserved metazoan MAB21 proteins are implicated in cell fate decisions and share considerable sequence homology with the cyclic GMP-AMP synthase. cGAS is the major innate immune sensor for cytosolic DNA and produces the second messenger 2'-5', 3'-5' cyclic GMP-AMP. Little is known about the structure and biochemical function of other proteins of the cGAS-MAB21 subfamily, such as MAB21L1, MAB21L2 and MAB21L3. We have determined the crystal structure of human full-length MAB21L1. Our analysis reveals high structural conservation between MAB21L1 and cGAS but also uncovers important differences. Although monomeric in solution, MAB21L1 forms a highly symmetric double-pentameric oligomer in the crystal, raising the possibility that oligomerization could be a feature of MAB21L1. In the crystal, MAB21L1 is in an inactive conformation requiring a conformational change - similar to cGAS - to develop any nucleotidyltransferase activity. Co-crystallization with NTP identified a putative ligand binding site of MAB21 proteins that corresponds to the DNA binding site of cGAS. Finally, we offer a structure-based explanation for the effects of MAB21L2 mutations in patients with eye malformations. The underlying residues participate in fold-stabilizing interaction networks and mutations destabilize the protein. In summary, we provide a first structural framework for MAB21 proteins.
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| - | Structural and biochemical characterization of the cell fate determining nucleotidyltransferase fold protein MAB21L1.,de Oliveira Mann CC, Kiefersauer R, Witte G, Hopfner KP Sci Rep. 2016 Jun 8;6:27498. doi: 10.1038/srep27498. PMID:27271801<ref>PMID:27271801</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5eog" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hopfner, K P]] | + | [[Category: Hopfner K-P]] |
| - | [[Category: Mann, C C.de Oliveira]]
| + | [[Category: Witte G]] |
| - | [[Category: Witte, G]] | + | [[Category: De Oliveira Mann CC]] |
| - | [[Category: Nucleotidyltransferase fold protein]] | + | |
| - | [[Category: Transferase]]
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