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| | <StructureSection load='6w2q' size='340' side='right'caption='[[6w2q]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6w2q' size='340' side='right'caption='[[6w2q]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6w2q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W2Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W2Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W2Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w2q OCA], [http://pdbe.org/6w2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w2q RCSB], [http://www.ebi.ac.uk/pdbsum/6w2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w2q ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w2q OCA], [https://pdbe.org/6w2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w2q RCSB], [https://www.ebi.ac.uk/pdbsum/6w2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w2q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Synthetic construct sequences]]
| + | [[Category: Baker D]] |
| - | [[Category: Baker, D]] | + | [[Category: Bick MJ]] |
| - | [[Category: Bick, M J]] | + | [[Category: Brunette TJ]] |
| - | [[Category: Brunette, T J]] | + | |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Computational design]]
| + | |
| - | [[Category: Designed helical repeat]]
| + | |
| - | [[Category: Helical bundle]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The ability to precisely design large proteins with diverse shapes would enable applications ranging from the design of protein binders that wrap around their target to the positioning of multiple functional sites in specified orientations. We describe a protein backbone design method for generating a wide range of rigid fusions between helix-containing proteins and use it to design 75,000 structurally unique junctions between monomeric and homo-oligomeric de novo designed and ankyrin repeat proteins (RPs). Of the junction designs that were experimentally characterized, 82% have circular dichroism and solution small-angle X-ray scattering profiles consistent with the design models and are stable at 95 degrees C. Crystal structures of four designed junctions were in close agreement with the design models with rmsds ranging from 0.9 to 1.6 A. Electron microscopic images of extended tetrameric structures and approximately 10-nm-diameter "L" and "V" shapes generated using the junctions are close to the design models, demonstrating the control the rigid junctions provide for protein shape sculpting over multiple nanometer length scales.
Modular repeat protein sculpting using rigid helical junctions.,Brunette TJ, Bick MJ, Hansen JM, Chow CM, Kollman JM, Baker D Proc Natl Acad Sci U S A. 2020 Apr 3. pii: 1908768117. doi:, 10.1073/pnas.1908768117. PMID:32245816[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brunette TJ, Bick MJ, Hansen JM, Chow CM, Kollman JM, Baker D. Modular repeat protein sculpting using rigid helical junctions. Proc Natl Acad Sci U S A. 2020 Apr 3. pii: 1908768117. doi:, 10.1073/pnas.1908768117. PMID:32245816 doi:http://dx.doi.org/10.1073/pnas.1908768117
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