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| ==NMR STRUCTURE OF Gln25-RIBONUCLEASE T1, 24 STRUCTURES== | | ==NMR STRUCTURE OF Gln25-RIBONUCLEASE T1, 24 STRUCTURES== |
- | <StructureSection load='1iyy' size='340' side='right'caption='[[1iyy]], [[NMR_Ensembles_of_Models | 24 NMR models]]' scene=''> | + | <StructureSection load='1iyy' size='340' side='right'caption='[[1iyy]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[1iyy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspoz Aspoz]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1IYY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1iyy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IYY FirstGlance]. <br> |
- | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ygw|1ygw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
- | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iyy OCA], [https://pdbe.org/1iyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iyy RCSB], [https://www.ebi.ac.uk/pdbsum/1iyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iyy ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1iyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iyy OCA], [http://pdbe.org/1iyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1iyy RCSB], [http://www.ebi.ac.uk/pdbsum/1iyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1iyy ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/RNT1_ASPOR RNT1_ASPOR] |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| ==See Also== | | ==See Also== |
| *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
- | *[[Temp|Temp]] | |
| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Aspoz]] | + | [[Category: Aspergillus oryzae]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Hatano, K]] | + | [[Category: Hatano K]] |
- | [[Category: Kojima, M]] | + | [[Category: Kojima M]] |
- | [[Category: Suzuki, E]] | + | [[Category: Suzuki E]] |
- | [[Category: Takahashi, K]] | + | [[Category: Takahashi K]] |
- | [[Category: Tanokura, M]] | + | [[Category: Tanokura M]] |
- | [[Category: Endonuclease]]
| + | |
- | [[Category: Endoribonuclease]]
| + | |
- | [[Category: Hydrolase]]
| + | |
- | [[Category: Ribonuclease]]
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| Structural highlights
Function
RNT1_ASPOR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ribonuclease (RNase) T1 is a guanyloribonuclease, having two isozymes in nature, Gln25- and Lys25-RNase T1. Between these two isozymes, there is no difference in catalytic activity and three-dimensional structure; however, Lys25-RNase T1 is slightly more stable than Gln25-RNase T1. Recently, it has been suggested that the existence of a salt bridge between Lys25 and Asp29/Glu31 in Lys25-RNase T1 contributes to the stability. To elucidate the effects of the replacement of Lys25 with a Gln on the conformation and microenvironments of RNase T1 in detail, the three-dimensional solution structure of Gln25-RNase T1 was determined by simulated-annealing calculations. As a result, the topology of the overall folding was shown to be very similar to that of the Lys25-isozyme except for some differences. In particular, there were two differences in the property of torsion angles of the two disulfide bonds and the conformations of the residues 11-13, 63-66, and 92-93. With regard to the residues 11-13, the lack of the above-mentioned salt bridge in Gln25-RNase T1 was thought to induce the conformational difference of this segment as compared with the Lys25-isozyme. Furthermore, it was proposed that the perturbation of this segment might transfer to the residues 92-93 via the two disulfide bonds.
Determination of the NMR structure of Gln25-ribonuclease T1.,Hatano K, Kojima M, Suzuki E, Tanokura M, Takahashi K Biol Chem. 2003 Aug;384(8):1173-83. PMID:12974386[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hatano K, Kojima M, Suzuki E, Tanokura M, Takahashi K. Determination of the NMR structure of Gln25-ribonuclease T1. Biol Chem. 2003 Aug;384(8):1173-83. PMID:12974386
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