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| | ==Solution structure of a zinc-binding domain from the Junin virus envelope glycoprotein== | | ==Solution structure of a zinc-binding domain from the Junin virus envelope glycoprotein== |
| - | <StructureSection load='2l0z' size='340' side='right'caption='[[2l0z]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | + | <StructureSection load='2l0z' size='340' side='right'caption='[[2l0z]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l0z]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2L0Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l0z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Argentinian_mammarenavirus Argentinian mammarenavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 21 models</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2l0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0z OCA], [http://pdbe.org/2l0z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0z RCSB], [http://www.ebi.ac.uk/pdbsum/2l0z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0z OCA], [https://pdbe.org/2l0z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0z RCSB], [https://www.ebi.ac.uk/pdbsum/2l0z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GLYC_JUNIN GLYC_JUNIN]] Stable signal peptide (SSP) is cleaved but is apparently retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational cleavage of GP1 and GP2, glycoprotein transport to the cell plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion (By similarity). Glycoprotein G1 mediates virus attachment to host TFRC. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis.<ref>PMID:19548229</ref> Glycoprotein G2 is a class I viral fusion protein, that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversable conformational changes induced upon acidification in the endosome (By similarity). | + | [https://www.uniprot.org/uniprot/GLYC_JUNIN GLYC_JUNIN] Stable signal peptide (SSP) is cleaved but is apparently retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational cleavage of GP1 and GP2, glycoprotein transport to the cell plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion (By similarity). Glycoprotein G1 mediates virus attachment to host TFRC. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis.<ref>PMID:19548229</ref> Glycoprotein G2 is a class I viral fusion protein, that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversable conformational changes induced upon acidification in the endosome (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Argentinian mammarenavirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Briknarova, K]] | + | [[Category: Briknarova K]] |
| - | [[Category: Nunberg, J H]] | + | [[Category: Nunberg JH]] |
| - | [[Category: York, J]] | + | [[Category: York J]] |
| - | [[Category: Arenavirus]]
| + | |
| - | [[Category: Gpc]]
| + | |
| - | [[Category: Junin virus]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Virus envelope glycoprotein]]
| + | |
| - | [[Category: Zinc-binding domain]]
| + | |
| Structural highlights
Function
GLYC_JUNIN Stable signal peptide (SSP) is cleaved but is apparently retained as the third component of the GP complex. The SSP is required for efficient glycoprotein expression, post-translational cleavage of GP1 and GP2, glycoprotein transport to the cell plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion (By similarity). Glycoprotein G1 mediates virus attachment to host TFRC. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis.[1] Glycoprotein G2 is a class I viral fusion protein, that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversable conformational changes induced upon acidification in the endosome (By similarity).
Publication Abstract from PubMed
Arenaviruses cause acute hemorrhagic fevers with high mortality. Entry of the virus into the host cell is mediated by the viral envelope glycoprotein, GPC. In contrast to other class I viral envelope glycoproteins, the mature GPC complex contains a cleaved stable signal peptide (SSP) in addition to the canonical receptor-binding (G1) and transmembrane fusion (G2) subunits. SSP is critical for intracellular transport of the GPC complex to the cell surface and for its membrane-fusion activity. Previous studies have suggested that SSP is retained in GPC through interaction with a zinc-binding domain (ZBD) in the cytoplasmic tail of G2. Here we used NMR spectroscopy to determine the structure of Junin virus (JUNV) ZBD (G2 residues 445-485) and investigate its interaction with a conserved Cys residue (Cys-57) in SSP. We show that JUNV ZBD displays a novel fold containing two zinc ions. One zinc ion is coordinated by His-447, His-449, Cys-455, and His-485. The second zinc ion is coordinated by His-459, Cys-467, and Cys-469 and readily accepts Cys-57 from SSP as the fourth ligand. Our studies describe the structural basis for retention of the unique SSP subunit and suggest a mechanism whereby SSP is positioned in the GPC complex to modulate pH-dependent membrane fusion.
Structure of a Zinc-binding Domain in the Junin Virus Envelope Glycoprotein.,Briknarova K, Thomas CJ, York J, Nunberg JH J Biol Chem. 2011 Jan 14;286(2):1528-36. Epub 2010 Nov 10. PMID:21068387[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Martinez MG, Forlenza MB, Candurra NA. Involvement of cellular proteins in Junin arenavirus entry. Biotechnol J. 2009 Jun;4(6):866-70. doi: 10.1002/biot.200800357. PMID:19548229 doi:http://dx.doi.org/10.1002/biot.200800357
- ↑ Briknarova K, Thomas CJ, York J, Nunberg JH. Structure of a Zinc-binding Domain in the Junin Virus Envelope Glycoprotein. J Biol Chem. 2011 Jan 14;286(2):1528-36. Epub 2010 Nov 10. PMID:21068387 doi:10.1074/jbc.M110.166025
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