6wpi
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6wpi is ON HOLD Authors: Zhang, J., Qiu, C., Hall, T.M.T. Description: Crystal structure of Nop9 in complex with ITS1 RNA [[Category: Unreleased St...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Nop9 in complex with ITS1 RNA== | |
| + | <StructureSection load='6wpi' size='340' side='right'caption='[[6wpi]], [[Resolution|resolution]] 3.02Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6wpi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPI FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TCE:3,3,3-PHOSPHANETRIYLTRIPROPANOIC+ACID'>TCE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpi OCA], [https://pdbe.org/6wpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpi RCSB], [https://www.ebi.ac.uk/pdbsum/6wpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpi ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nop9 is an essential factor in the processing of pre-ribosomal RNA. Its absence in yeast is lethal, and defects in the human ortholog are associated with breast cancer, autoimmunity, and learning/language impairment. PUF family RNA-binding proteins are best known for sequence-specific RNA recognition, and most contain eight alpha-helical repeats that bind to the RNA bases of single-stranded RNA. Nop9 is an unusual member of this family in that it contains eleven repeats and recognizes both RNA structure and sequence. Here we report a crystal structure of Saccharomyces cerevisiae Nop9 in complex with its target RNA within the 20S pre-ribosomal RNA. This structure reveals that Nop9 brings together a C-terminal module recognizing the 5' single-stranded region of the RNA and a bifunctional N-terminal module recognizing the central double-stranded stem region. We further show that the 3' single-stranded region of the 20S target RNA adds sequence-independent binding energy to the RNA-Nop9 interaction. Both the N- and C-terminal modules retain the characteristic sequence-specific recognition of PUF proteins but the N-terminal module has also evolved a distinct interface, which allows Nop9 to recognize either single-stranded RNA sequences or RNAs with a combination of single-stranded and structured elements. | ||
| - | + | Nop9 recognizes structured and single-stranded RNA elements of pre-ribosomal RNA.,Zhang J, Teramoto T, Qiu C, Wine RN, Gonzalez LE, Baserga SJ, Hall TM RNA. 2020 May 5. pii: rna.075416.120. doi: 10.1261/rna.075416.120. PMID:32371454<ref>PMID:32371454</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6wpi" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hall | + | == References == |
| - | [[Category: Zhang | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Hall TMT]] | ||
| + | [[Category: Qiu C]] | ||
| + | [[Category: Zhang J]] | ||
Current revision
Crystal structure of Nop9 in complex with ITS1 RNA
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