1z8u

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(New page: 200px<br /> <applet load="1z8u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z8u, resolution 2.4&Aring;" /> '''Crystal structure of...)
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[[Image:1z8u.gif|left|200px]]<br />
 
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<applet load="1z8u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1z8u, resolution 2.4&Aring;" />
 
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'''Crystal structure of oxidized alpha hemoglobin bound to AHSP'''<br />
 
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==Overview==
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==Crystal structure of oxidized alpha hemoglobin bound to AHSP==
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The synthesis of haemoglobin A (HbA) is exquisitely coordinated during, erythrocyte development to prevent damaging effects from individual alpha-, and beta-subunits. The alpha-haemoglobin-stabilizing protein (AHSP) binds, alpha-haemoglobin (alphaHb), inhibits the ability of alphaHb to generate, reactive oxygen species and prevents its precipitation on exposure to, oxidant stress. The structure of AHSP bound to ferrous alphaHb is thought, to represent a transitional complex through which alphaHb is converted to, a non-reactive, hexacoordinate ferric form. Here we report the crystal, structure of this ferric alphaHb-AHSP complex at 2.4 A resolution. Our, findings reveal a striking bis-histidyl configuration in which both the, proximal and the distal histidines coordinate the haem iron atom. To, attain this unusual conformation, segments of alphaHb undergo drastic, structural rearrangements, including the repositioning of several, alpha-helices. Moreover, conversion to the ferric bis-histidine, configuration strongly and specifically inhibits redox chemistry catalysis, and haem loss from alphaHb. The observed structural changes, which impair, the chemical reactivity of haem iron, explain how AHSP stabilizes alphaHb, and prevents its damaging effects in cells.
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<StructureSection load='1z8u' size='340' side='right'caption='[[1z8u]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1z8u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z8U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z8u OCA], [https://pdbe.org/1z8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z8u RCSB], [https://www.ebi.ac.uk/pdbsum/1z8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z8u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AHSP_HUMAN AHSP_HUMAN] Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.<ref>PMID:12066189</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/1z8u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z8u ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Z8U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z8U OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of oxidized alpha-haemoglobin bound to AHSP reveals a protective mechanism for haem., Feng L, Zhou S, Gu L, Gell DA, Mackay JP, Weiss MJ, Gow AJ, Shi Y, Nature. 2005 Jun 2;435(7042):697-701. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15931225 15931225]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Feng, L.]]
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[[Category: Feng L]]
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[[Category: Gell, D.A.]]
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[[Category: Gell DA]]
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[[Category: Gow, A.J.]]
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[[Category: Gow AJ]]
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[[Category: Gu, L.]]
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[[Category: Gu L]]
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[[Category: Mackay, J.P.]]
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[[Category: Mackay JP]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: Weiss, M.J.]]
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[[Category: Weiss MJ]]
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[[Category: Zhou, S.]]
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[[Category: Zhou S]]
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[[Category: HEM]]
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[[Category: ahsp]]
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[[Category: alpha haemoglobin]]
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[[Category: interaction]]
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[[Category: oxidation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:27:56 2007''
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Current revision

Crystal structure of oxidized alpha hemoglobin bound to AHSP

PDB ID 1z8u

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