|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Three-dimensional structure of the cyclotide Cter M== | | ==Three-dimensional structure of the cyclotide Cter M== |
| - | <StructureSection load='2lam' size='340' side='right'caption='[[2lam]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lam' size='340' side='right'caption='[[2lam]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lam]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2LAM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lam]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clitoria_ternatea Clitoria ternatea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LAM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2lam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lam OCA], [http://pdbe.org/2lam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lam RCSB], [http://www.ebi.ac.uk/pdbsum/2lam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lam ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lam OCA], [https://pdbe.org/2lam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lam RCSB], [https://www.ebi.ac.uk/pdbsum/2lam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lam ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CYCM_CLITE CYCM_CLITE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 19: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Clitoria ternatea]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Colgrave, M L]] | + | [[Category: Colgrave ML]] |
| - | [[Category: Craik, D J]] | + | [[Category: Craik DJ]] |
| - | [[Category: Daly, N L]] | + | [[Category: Daly NL]] |
| - | [[Category: Lyons, R E]] | + | [[Category: Lyons RE]] |
| - | [[Category: Poth, A G]] | + | [[Category: Poth AG]] |
| - | [[Category: Antiviral protein]]
| + | |
| - | [[Category: Insecticidal peptide]]
| + | |
| Structural highlights
Function
CYCM_CLITE
Publication Abstract from PubMed
Cyclotides are plant-derived proteins that have a unique cyclic cystine knot topology and are remarkably stable. Their natural function is host defense, but they have a diverse range of pharmaceutically important activities, including uterotonic activity and anti-HIV activity, and have also attracted recent interest as templates in drug design. Here we report an unusual biosynthetic origin of a precursor protein of a cyclotide from the butterfly pea, Clitoria ternatea, a representative member of the Fabaceae plant family. Unlike all previously reported cyclotides, the domain corresponding to the mature cyclotide from this Fabaceae plant is embedded within an albumin precursor protein. We confirmed the expression and correct processing of the cyclotide encoded by the Cter M precursor gene transcript following extraction from C. ternatea leaf and sequencing by tandem mass spectrometry. The sequence was verified by direct chemical synthesis and the peptide was found to adopt a classic knotted cyclotide fold as determined by NMR spectroscopy. Seven additional cyclotide sequences were also identified from C. ternatea leaf and flower, five of which were unique. Cter M displayed insecticidal activity against the cotton budworm Helicoverpa armigera and bound to phospholipid membranes, suggesting its activity is modulated by membrane disruption. The Fabaceae is the third largest family of flowering plants and many Fabaceous plants are of huge significance for human nutrition. Knowledge of Fabaceae cyclotide gene transcripts should enable the production of modified cyclotides in crop plants for a variety of agricultural or pharmaceutical applications, including plant-produced designer peptide drugs.
From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes.,Poth AG, Colgrave ML, Lyons RE, Daly NL, Craik DJ Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10127-32. Epub 2011 May 18. PMID:21593408[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Poth AG, Colgrave ML, Lyons RE, Daly NL, Craik DJ. From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10127-32. Epub 2011 May 18. PMID:21593408 doi:10.1073/pnas.1103660108
|
