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5fia

From Proteopedia

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<StructureSection load='5fia' size='340' side='right'caption='[[5fia]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='5fia' size='340' side='right'caption='[[5fia]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5fia]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FIA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FIA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5fia]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FIA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpymg_00644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fia OCA], [http://pdbe.org/5fia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fia RCSB], [http://www.ebi.ac.uk/pdbsum/5fia PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fia ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fia OCA], [https://pdbe.org/5fia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fia RCSB], [https://www.ebi.ac.uk/pdbsum/5fia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fia ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5ZXU7_LEGPH Q5ZXU7_LEGPH]
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Legionella pneumophila is a causative agent of a severe pneumonia, known as Legionnaires' disease. Legionella's pathogenicity is mediated by specific virulence factors, called bacterial effectors, which are injected into the invaded host cell by the bacterial Type IV Secretion System. Bacterial effectors are involved in complex interactions with the components of the host cell immune and signaling pathways, that eventually lead to bacterial survival and replication inside the mammalian cell. Structural and functional studies of bacterial effectors are therefore crucial for elucidating the mechanisms of Legionella virulence. Here we describe the crystal structure of the LpiR1 (Lpg0634) effector protein and investigate the effects of its overexpression in mammalian cells. LpiR1 is an alpha-helical protein, that consists of two similar domains aligned in an antiparallel fashion. The hydrophilic cleft between the domains might serve as a binding site for a potential host cell interaction partner. LpiR1 binds phosphate group at a conserved site and is stabilized by Mn2+, Ca2+ or Mg2+ ions. When overexpressed in mammalian cells, a GFP-LpiR1 fusion protein is localized in the cytoplasm. Intracellular signaling antibody array analysis revealed small changes in the phosphorylation state of several components of the Akt signaling pathway in HEK293T cells overexpressing LpiR1.
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Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila.,Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543<ref>PMID:27226543</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5fia" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Legph]]
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[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
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[[Category: Beyrakhova, K]]
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[[Category: Beyrakhova K]]
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[[Category: Cygler, M]]
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[[Category: Cygler M]]
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[[Category: Straaten, K van]]
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[[Category: Van Straaten K]]
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[[Category: Bacterial effector]]
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[[Category: Internal repeat]]
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[[Category: Legionella pneumophila]]
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[[Category: Signaling protein]]
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Current revision

Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila

PDB ID 5fia

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