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Nucleoside triphosphatase
From Proteopedia
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| - | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
| + | <StructureSection load='4a5a' size='350' side='right' caption='NTPase 2 tetramer complex with AMPPNP and Mg++ ion (green) (PDB entry [[4a5a]])' scene=''> | ||
== Function == | == Function == | ||
| - | '''Nucleoside triphosphatase''' or '''nucleoside triphosphate diphosphohydrolase''' (NTPase) | + | '''Nucleoside triphosphatase''' or '''nucleoside triphosphate diphosphohydrolase''' (NTPase) is responsible for degradation of nucleotides to their monophosphate form. NTPase is found in mammals and in pathogenic microbes. In mammals NTPase hs a crucial role in regulation of purinergic signalling by hydrolysis of extracellular nucleotides. The function of NTPase in pathogens is still unknown<ref>PMID:24115522</ref>. |
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== Relevance == | == Relevance == | ||
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| + | The modulation of NTPase activity sems a good therapeutic method for regulating the concentration of ATP. High ATP concentration has been shown to be involved in various disorders in the CNS including brain injury, ischemia, neuro-inflammation, epilepsy, neuropathic pain and migraine<ref>PMID:25694082</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
| + | ''Toxoplasma gondii'' <snapshot name='84/844927/Cv1/3'>NTPase 2 is dimer of dimers</snapshot> (PDB code [[4a5a]]). The 3D structure of the complex between NTPase 2 and the ATP analog AMPPNP shows the NTPase structure composed of two domains. Domain I contains the N-terminal and C-terminal and domain II the core residues. The structure contains 7 Cys-Cys bonds one of which located between domain I and II and reaching the diametrically positioned monomer was found by mutational analysis to be responsible for activation. The ATP analog - AMPPNP - is located in a cleft and forms interactions with domain I and domain II<ref>PMID:22130673</ref>. | ||
| + | *<snapshot name='84/844927/Cv1/5'>Test1</snapshot> | ||
| + | *<snapshot name='84/844927/Cv1/6'>Test2</snapshot> | ||
| + | *<snapshot name='84/844927/Cv1/8'>Test3</snapshot> | ||
| - | + | </SX> | |
| - | </ | + | |
==3D structures of nucleoside triphosphatase== | ==3D structures of nucleoside triphosphatase== | ||
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**[[6dt3]] - Nudi - ''Klebsiella pneumoniae'' <br /> | **[[6dt3]] - Nudi - ''Klebsiella pneumoniae'' <br /> | ||
| + | **[[3oga]], [[3n77]] - Nudi - ''Salmonella enterica'' <br /> | ||
*Nucleoside triphosphatase cancer-related | *Nucleoside triphosphatase cancer-related | ||
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**[[3s86]] - NTPase - ''Thermotoga maritima'' <br /> | **[[3s86]] - NTPase - ''Thermotoga maritima'' <br /> | ||
| - | + | **[[3agr]] - NTPase - ''Neospora caninum'' <br /> | |
| + | **[[7aoh]] - NTPase I in transcribing complex - Vaccinia virus – Cryo EM <br /> | ||
| + | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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