6v93
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of DNA Polymerase Zeta/DNA/dNTP Ternary Complex== | |
| + | <StructureSection load='6v93' size='340' side='right'caption='[[6v93]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6v93]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V93 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v93 OCA], [https://pdbe.org/6v93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v93 RCSB], [https://www.ebi.ac.uk/pdbsum/6v93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v93 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DPOZ_YEAST DPOZ_YEAST] Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion.<ref>PMID:11316789</ref> <ref>PMID:16452144</ref> <ref>PMID:2676986</ref> <ref>PMID:8658138</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA polymerase zeta (Polzeta) belongs to the same B-family as high-fidelity replicative polymerases, yet is specialized for the extension reaction in translesion DNA synthesis (TLS). Despite its importance in TLS, the structure of Polzeta is unknown. We present cryo-EM structures of the Saccharomyces cerevisiae Polzeta holoenzyme in the act of DNA synthesis (3.1 A) and without DNA (4.1 A). Polzeta displays a pentameric ring-like architecture, with catalytic Rev3, accessory Pol31' Pol32 and two Rev7 subunits forming an uninterrupted daisy chain of protein-protein interactions. We also uncover the features that impose high fidelity during the nucleotide-incorporation step and those that accommodate mismatches and lesions during the extension reaction. Collectively, we decrypt the molecular underpinnings of Polzeta's role in TLS and provide a framework for new cancer therapeutics. | ||
| - | + | Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.,Malik R, Kopylov M, Gomez-Llorente Y, Jain R, Johnson RE, Prakash L, Prakash S, Ubarretxena-Belandia I, Aggarwal AK Nat Struct Mol Biol. 2020 Oct;27(10):913-924. doi: 10.1038/s41594-020-0476-7., Epub 2020 Aug 17. PMID:32807989<ref>PMID:32807989</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6v93" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Aggarwal AK]] | ||
| + | [[Category: Jain R]] | ||
| + | [[Category: Kopylov M]] | ||
| + | [[Category: Malik R]] | ||
| + | [[Category: Ubarrextena-Belandia I]] | ||
Current revision
Structure of DNA Polymerase Zeta/DNA/dNTP Ternary Complex
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