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| | ==Structure of insect-specific sodium channel toxin mu-Dc1a== | | ==Structure of insect-specific sodium channel toxin mu-Dc1a== |
| - | <StructureSection load='2mi5' size='340' side='right'caption='[[2mi5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mi5' size='340' side='right'caption='[[2mi5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mi5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Digca Digca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MI5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mi5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Diguetia_canities Diguetia canities]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2mi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi5 OCA], [http://pdbe.org/2mi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi5 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi5 OCA], [https://pdbe.org/2mi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi5 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TXI92_DIGCA TXI92_DIGCA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Digca]] | + | [[Category: Diguetia canities]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bende, N S]] | + | [[Category: Bende NS]] |
| - | [[Category: King, G F]] | + | [[Category: King GF]] |
| - | [[Category: Mobli, M]] | + | [[Category: Mobli M]] |
| - | [[Category: Dtx9 2]]
| + | |
| - | [[Category: Gating modifier]]
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| - | [[Category: Inhibitor cystine knot]]
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| - | [[Category: Insecticidal toxin]]
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| - | [[Category: Knottin]]
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| - | [[Category: Non-uniform sampling]]
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| - | [[Category: Sodium channel]]
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| - | [[Category: Spider venom]]
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| - | [[Category: Toxin]]
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| - | [[Category: Voltage-sensor]]
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| Structural highlights
Function
TXI92_DIGCA
Publication Abstract from PubMed
beta-Diguetoxin-Dc1a (Dc1a) is a toxin from the desert bush spider Diguetia canities that incapacitates insects at concentrations that are non-toxic to mammals. Dc1a promotes opening of German cockroach voltage-gated sodium (Nav) channels (BgNav1), whereas human Nav channels are insensitive. Here, by transplanting commonly targeted S3b-S4 paddle motifs within BgNav1 voltage sensors into Kv2.1, we find that Dc1a interacts with the domain II voltage sensor. In contrast, Dc1a has little effect on sodium currents mediated by PaNav1 channels from the American cockroach even though their domain II paddle motifs are identical. When exploring regions responsible for PaNav1 resistance to Dc1a, we identified two residues within the BgNav1 domain II S1-S2 loop that when mutated to their PaNav1 counterparts drastically reduce toxin susceptibility. Overall, our results reveal a distinct region within insect Nav channels that helps determine Dc1a sensitivity, a concept that will be valuable for the design of insect-selective insecticides.
A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a.,Bende NS, Dziemborowicz S, Mobli M, Herzig V, Gilchrist J, Wagner J, Nicholson GM, King GF, Bosmans F Nat Commun. 2014 Jul 11;5:4350. doi: 10.1038/ncomms5350. PMID:25014760[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bende NS, Dziemborowicz S, Mobli M, Herzig V, Gilchrist J, Wagner J, Nicholson GM, King GF, Bosmans F. A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a. Nat Commun. 2014 Jul 11;5:4350. doi: 10.1038/ncomms5350. PMID:25014760 doi:http://dx.doi.org/10.1038/ncomms5350
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