2mj0
From Proteopedia
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==Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia== | ==Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia== | ||
| - | <StructureSection load='2mj0' size='340' side='right'caption='[[2mj0 | + | <StructureSection load='2mj0' size='340' side='right'caption='[[2mj0]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2mj0]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2mj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_kaouthia Naja kaouthia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJ0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mj0 OCA], [https://pdbe.org/2mj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mj0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mj0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/3NO2_NAJKA 3NO2_NAJKA] Neurotoxin that irreversibly inhibits nicotinic acetylcholine receptors (nAChR) and allosterically interacts with muscarinic acetylcholine receptors (mAChR) (PubMed:11279130, PubMed:19682302). The loop II is involved in the interaction of this toxin with nAChR and mAChR (PubMed:26242733, PubMed:27343701). On nAChR, it acts as a competitive antagonist (muscle-type and alpha-7/CHRNA7) with IC(50) values in the micromolar range (PubMed:11279130, PubMed:27343701). On mAChR, in presence of ACh, it partially inhibits the effect of acetylcholine (ACh) (allosteric antagonist), whereas in the absence of ACh, it activates the receptor (allosteric agonist) (PubMed:19682302). It also shows a very weak inhibition of GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1 and GABRB3 and GABRG2) subunits (10 uM inhibit 31% current) (PubMed:26221036). In vivo, is nonlethal to mice at concentrations up to 20 mg/kg, but exerts a myorelaxant effect, induces a dose-dependent decrease in blood pressure and an increase in heart rate in mice and rats (PubMed:15581687, PubMed:17371532).<ref>PMID:11279130</ref> <ref>PMID:15581687</ref> <ref>PMID:17371532</ref> <ref>PMID:19682302</ref> <ref>PMID:26221036</ref> <ref>PMID:27343701</ref> |
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Naja kaouthia]] |
| - | [[Category: Lyukmanova | + | [[Category: Lyukmanova EN]] |
| - | [[Category: Paramonov | + | [[Category: Paramonov AS]] |
| - | [[Category: Shenkarev | + | [[Category: Shenkarev ZO]] |
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Current revision
Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia
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