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| | <StructureSection load='5glv' size='340' side='right'caption='[[5glv]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='5glv' size='340' side='right'caption='[[5glv]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5glv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Parasitic_roundworm Parasitic roundworm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GLV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5glv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxascaris_leonina Toxascaris leonina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GLV FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5gm0|5gm0]], [[5glw|5glw]], [[5glt|5glt]], [[5glu|5glu]], [[5glz|5glz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5glv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5glv OCA], [http://pdbe.org/5glv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5glv RCSB], [http://www.ebi.ac.uk/pdbsum/5glv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5glv ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5glv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5glv OCA], [https://pdbe.org/5glv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5glv RCSB], [https://www.ebi.ac.uk/pdbsum/5glv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5glv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A1L1QJZ7_TOXLN A0A1L1QJZ7_TOXLN] |
| - | Toxascaris leonina galectin (Tl-gal) is a galectin-9 homologue protein isolated from an adult worm of the canine gastrointestinal nematode parasite, and Tl-gal-vaccinated challenge can inhibit inflammation in inflammatory bowel disease-induced mice. We determined the first X-ray structures of full-length Tl-gal complexes with carbohydrates (lactose, N-acetyllactosamine, lacto-N-tetraose, sialyllactose, and glucose). Bonds were formed on concave surfaces of both carbohydrate recognition domains (CRDs) in Tl-gal. All binding sites were found in the HXXXR and WGXEER motifs. Charged Arg61/Arg196 and Glu80/Glu215 on the conserved motif of Tl-gal N-terminal CRD and C-terminal CRD are critical amino acids for recognizing carbohydrate binding, and the residues can affect protein folding and structure. The polar amino acids His, Asn, and Trp are also important residues for the interaction with carbohydrates through hydrogen bonding. Hemagglutination activities of Tl-gal were inhibited by interactions with carbohydrates and mutations. We found that the mutation of Tl-gal (E80A/E215A) at the carbohydrate binding region induced protein aggregation and could be caused in many diseases. The short linker region between the N-terminal and C-terminal CRDs of Tl-gal was very stable against proteolysis and maintained its biological activity. This structural information is expected to elucidate the carbohydrate recognition mechanism of Tl-gal and improve our understanding of anti-inflammatory mediators and modulators of immune response.
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| - | Structural Basis for Carbohydrate Recognition and Anti-inflammatory Modulation by Gastrointestinal Nematode Parasite Toxascaris leonina Galectin.,Hwang EY, Jeong MS, Park SK, Ha SC, Yu HS, Jang SB J Biol Chem. 2016 Dec 2;291(49):25326-25338. Epub 2016 Oct 14. PMID:27742836<ref>PMID:27742836</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 5glv" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Galectin 3D structures|Galectin 3D structures]] | | *[[Galectin 3D structures|Galectin 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Parasitic roundworm]] | |
| - | [[Category: Hwang, E Y]] | |
| - | [[Category: Jang, S B]] | |
| - | [[Category: Carbohydrate]] | |
| - | [[Category: Crystal structure]] | |
| - | [[Category: Galectin]] | |
| - | [[Category: Sugar binding protein]] | |
| | [[Category: Toxascaris leonina]] | | [[Category: Toxascaris leonina]] |
| | + | [[Category: Hwang EY]] |
| | + | [[Category: Jang SB]] |
