6tku

<table><tr><td colspan='2'>[[6tku]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_phage_kp32 Klebsiella phage kp32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TKU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TKU FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">38 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=674082 Klebsiella phage KP32])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tku OCA], [http://pdbe.org/6tku PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tku RCSB], [http://www.ebi.ac.uk/pdbsum/6tku PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tku ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Capsule polysaccharide is a major virulence factor of Klebsiella pneumoniae, a nosocomial pathogen associated with a wide range of infections. It protects bacteria from harsh environmental conditions, immune system response, and phage infection. To access cell wall-located receptors, some phages possess tailspike depolymerases that degrade the capsular polysaccharide. Here, we present the crystal structure of a tailspike against Klebsiella, KP32gp38, whose primary sequence shares no similarity to other proteins of known structure. In the trimeric structure of KP32gp38, each chain contains a flexible N-terminal domain, a right-handed parallel beta helix domain and two beta sandwiches with carbohydrate binding features. The crystal structure and activity assays allowed us to locate the catalytic site. Also, our data provide experimental evidence of a branching architecture of depolymerases in KP32 Klebsiella viruses, as KP32gp38 displays nanomolar affinity to another depolymerase from the same phage, KP32gp37. Results provide a structural framework for enzyme engineering to produce serotype-broad-active enzyme complexes against K. pneumoniae.

Structural and Functional Studies of a Klebsiella Phage Capsule Depolymerase Tailspike: Mechanistic Insights into Capsular Degradation.,Squeglia F, Maciejewska B, Latka A, Ruggiero A, Briers Y, Drulis-Kawa Z, Berisio R Structure. 2020 May 6. pii: S0969-2126(20)30136-2. doi:, 10.1016/j.str.2020.04.015. PMID:32386574<ref>PMID:32386574</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Klebsiella phage kp32]]

[[Category: Berisio, R]]

[[Category: Squeglia, F]]

[[Category: Tail fiber branching system]]