2n5c

From Proteopedia

(Difference between revisions)

Current revision

Solution NMR structure of the lasso peptide chaxapeptin

Structural highlights

2n5c is a 1 chain structure with sequence from Streptomyces leeuwenhoekii. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR, 15 models
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0F7VRL1_STRLW

Publication Abstract from PubMed

Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique "lariat knot" structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549.

Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert.,Elsayed SS, Trusch F, Deng H, Raab A, Prokes I, Busarakam K, Asenjo JA, Andrews BA, van West P, Bull AT, Goodfellow M, Yi Y, Ebel R, Jaspars M, Rateb ME J Org Chem. 2015 Sep 24. PMID:26402731^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Elsayed SS, Trusch F, Deng H, Raab A, Prokes I, Busarakam K, Asenjo JA, Andrews BA, van West P, Bull AT, Goodfellow M, Yi Y, Ebel R, Jaspars M, Rateb ME. Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert. J Org Chem. 2015 Sep 24. PMID:26402731 doi:http://dx.doi.org/10.1021/acs.joc.5b01878

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n5c"

@@ Line 1: / Line 1: @@
 ==Solution NMR structure of the lasso peptide chaxapeptin==
-<StructureSection load='2n5c' size='340' side='right'caption='[[2n5c]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
+<StructureSection load='2n5c' size='340' side='right'caption='[[2n5c]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2n5c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_leeuwenhoekii Streptomyces leeuwenhoekii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2N5C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2n5c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_leeuwenhoekii Streptomyces leeuwenhoekii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N5C FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2n5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5c OCA], [http://pdbe.org/2n5c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n5c RCSB], [http://www.ebi.ac.uk/pdbsum/2n5c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5c ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5c OCA], [https://pdbe.org/2n5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n5c RCSB], [https://www.ebi.ac.uk/pdbsum/2n5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5c ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0F7VRL1_STRLW A0A0F7VRL1_STRLW]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 24: @@
 [[Category: Large Structures]]
 [[Category: Streptomyces leeuwenhoekii]]
-[[Category: Andrews, B A]]
+[[Category: Andrews BA]]
-[[Category: Asenjo, J A]]
+[[Category: Asenjo JA]]
-[[Category: Bull, A T]]
+[[Category: Bull AT]]
-[[Category: Busarakam, K]]
+[[Category: Busarakam K]]
-[[Category: Deng, H]]
+[[Category: Deng H]]
-[[Category: Ebel, R]]
+[[Category: Ebel R]]
-[[Category: Elsayed, S S]]
+[[Category: Elsayed SS]]
-[[Category: Goodfellow, M]]
+[[Category: Goodfellow M]]
-[[Category: Jaspars, M]]
+[[Category: Jaspars M]]
-[[Category: Prokes, I]]
+[[Category: Prokes I]]
-[[Category: Raab, A]]
+[[Category: Raab A]]
-[[Category: Rateb, M E]]
+[[Category: Rateb ME]]
-[[Category: Trusch, F]]
+[[Category: Trusch F]]
-[[Category: West, P van]]
+[[Category: Yi Y]]
-[[Category: Yi, Y]]
+[[Category: Van West P]]
-[[Category: Atacama desert]]
-[[Category: Cell invasion]]
-[[Category: Chaxapeptin]]
-[[Category: Genome mining]]
-[[Category: Ripp]]
-[[Category: Streptomyce]]

2n5c

From Proteopedia

Current revision

Solution NMR structure of the lasso peptide chaxapeptin

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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