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| | <StructureSection load='5h47' size='340' side='right'caption='[[5h47]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5h47' size='340' side='right'caption='[[5h47]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h47]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspor Aspor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H47 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H47 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h47]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae_RIB40 Aspergillus oryzae RIB40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H47 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FSW:(2S,3S,4R,5S,6R)-6-METHOXY-2-METHYL-5-METHYLSELANYL-OXANE-3,4-DIOL'>FSW</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eo7|5eo7]], [[5eo8|5eo8]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FSW:(2S,3S,4R,5S,6R)-6-METHOXY-2-METHYL-5-METHYLSELANYL-OXANE-3,4-DIOL'>FSW</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO090001000189 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=510516 ASPOR])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h47 OCA], [https://pdbe.org/5h47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h47 RCSB], [https://www.ebi.ac.uk/pdbsum/5h47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h47 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h47 OCA], [http://pdbe.org/5h47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h47 RCSB], [http://www.ebi.ac.uk/pdbsum/5h47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h47 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/LECF_ASPOR LECF_ASPOR] Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).<ref>PMID:12092808</ref> <ref>PMID:17383961</ref> <ref>PMID:19109923</ref> <ref>PMID:21790704</ref> <ref>PMID:27318092</ref> <ref>PMID:28041800</ref> <ref>PMID:28470344</ref> |
| - | Selenium-incorporated fucoses (seleno-fucoses) differing in the position of the seleno-substituent were synthesized and applied to the X-ray structural determination of a carbohydrate-lectin complex using single/multi-wavelength anomalous dispersion (SAD/MAD) phasing. The hydroxyl groups at the C-1, -2, -3 and -4 position of fucose were individually substituted with a methylseleno group via a transacetalization reaction using MeSeCH2OBn or by an SN2 reaction with TolSe- equivalents to afford the corresponding MeSe-fucose. The three-dimensional structures of a fucose-binding lectin complexed with several of these MeSe-fucoses have been determined by SAD/MAD phasing by utilizing the diffraction of selenium in the bound MeSe-fucoses.
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| - | | + | |
| - | Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing.,Shimabukuro J, Makyio H, Suzuki T, Nishikawa Y, Kawasaki M, Imamura A, Ishida H, Ando H, Kato R, Kiso M Bioorg Med Chem. 2016 Dec 19. pii: S0968-0896(16)31172-5. doi:, 10.1016/j.bmc.2016.12.021. PMID:28041800<ref>PMID:28041800</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| - | <div class="pdbe-citations 5h47" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspor]] | + | [[Category: Aspergillus oryzae RIB40]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kato, R]] | + | [[Category: Kato R]] |
| - | [[Category: Makyio, H]] | + | [[Category: Makyio H]] |
| - | [[Category: Nishikawa, Y]] | + | [[Category: Nishikawa Y]] |
| - | [[Category: Aspergillus oryzae]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Phaing]]
| + | |
| - | [[Category: Seleno-fucose]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
LECF_ASPOR Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).[1] [2] [3] [4] [5] [6] [7]
References
- ↑ Ishida H, Moritani T, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S. Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae. Biosci Biotechnol Biochem. 2002 May;66(5):1002-8. PMID:12092808 doi:10.1271/bbb.66.1002
- ↑ Matsumura K, Higashida K, Ishida H, Hata Y, Yamamoto K, Shigeta M, Mizuno-Horikawa Y, Wang X, Miyoshi E, Gu J, Taniguchi N. Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose. J Biol Chem. 2007 May 25;282(21):15700-8. PMID:17383961 doi:10.1074/jbc.M701195200
- ↑ Matsumura K, Higashida K, Hata Y, Kominami J, Nakamura-Tsuruta S, Hirabayashi J. Comparative analysis of oligosaccharide specificities of fucose-specific lectins from Aspergillus oryzae and Aleuria aurantia using frontal affinity chromatography. Anal Biochem. 2009 Mar 15;386(2):217-21. PMID:19109923 doi:10.1016/j.ab.2008.11.044
- ↑ Yamaki K, Yoshino S. Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE. Scand J Immunol. 2011 Nov;74(5):445-53. PMID:21790704 doi:10.1111/j.1365-3083.2011.02598.x
- ↑ Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R. Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092 doi:http://dx.doi.org/10.1016/j.bbrc.2016.06.069
- ↑ Shimabukuro J, Makyio H, Suzuki T, Nishikawa Y, Kawasaki M, Imamura A, Ishida H, Ando H, Kato R, Kiso M. Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing. Bioorg Med Chem. 2016 Dec 19. pii: S0968-0896(16)31172-5. doi:, 10.1016/j.bmc.2016.12.021. PMID:28041800 doi:http://dx.doi.org/10.1016/j.bmc.2016.12.021
- ↑ Ballal S, Belur S, Laha P, Roy S, Swamy BM, Inamdar SR. Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host-pathogen interactions leading to mycotic keratitis. Mol Cell Biochem. 2017 Oct;434(1-2):209-219. PMID:28470344 doi:10.1007/s11010-017-3050-9
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