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1a15

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(New page: 200px<br /> <applet load="1a15" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a15, resolution 2.2&Aring;" /> '''SDF-1ALPHA'''<br /> ...)
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[[Image:1a15.gif|left|200px]]<br />
 
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<applet load="1a15" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a15, resolution 2.2&Aring;" />
 
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'''SDF-1ALPHA'''<br />
 
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==Overview==
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==SDF-1ALPHA==
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Stromal cell-derived factor-1alpha (SDF-1alpha ) is a member of the, chemokine superfamily and functions as a growth factor and chemoattractant, through activation of CXCR4/LESTR/Fusin, a G protein-coupled receptor., This receptor also functions as a coreceptor for T-tropic, syncytium-inducing strains of HIV-1. SDF-1alpha antagonizes infectivity of, these strains by competing with gp120 for binding to the receptor. The, crystal structure of a variant SDF-1alpha ([N33A]SDF-1alpha ) prepared by, total chemical synthesis has been refined to 2.2-A resolution. Although, SDF-1alpha adopts a typical chemokine beta-beta-beta-alpha topology, the, packing of the alpha-helix against the beta-sheet is strikingly different., Comparison of SDF-1alpha with other chemokine structures confirms the, hypothesis that SDF-1alpha may be either an ancestral protein from which, all other chemokines evolved or the chemokine that is the least divergent, from a primordial chemokine. The structure of SDF-1alpha reveals a, positively charged surface ideal for binding to the negatively charged, extracellular loops of the CXCR4 HIV-1 coreceptor. This ionic, complementarity is likely to promote the interaction of the mobile, N-terminal segment of SDF-1alpha with interhelical sites of the receptor, resulting in a biological response.
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<StructureSection load='1a15' size='340' side='right'caption='[[1a15]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A15 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a15 OCA], [https://pdbe.org/1a15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a15 RCSB], [https://www.ebi.ac.uk/pdbsum/1a15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a15 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SDF1_HUMAN SDF1_HUMAN] Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.<ref>PMID:8752281</ref> <ref>PMID:11069075</ref> <ref>PMID:11859124</ref> <ref>PMID:16107333</ref> <ref>PMID:18802065</ref> <ref>PMID:19255243</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a1/1a15_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a15 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1A15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A15 OCA].
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*[[C-X-C motif chemokine 3D structures|C-X-C motif chemokine 3D structures]]
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*[[Stromal Derived Factor 1|Stromal Derived Factor 1]]
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==Reference==
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== References ==
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Crystal structure of chemically synthesized [N33A] stromal cell-derived factor 1alpha, a potent ligand for the HIV-1 "fusin" coreceptor., Dealwis C, Fernandez EJ, Thompson DA, Simon RJ, Siani MA, Lolis E, Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6941-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9618518 9618518]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Dealwis, C.G.]]
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</StructureSection>
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[[Category: Fernandez, E.J.]]
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[[Category: Homo sapiens]]
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[[Category: Lolis, E.]]
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[[Category: Large Structures]]
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[[Category: SO4]]
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[[Category: Dealwis CG]]
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[[Category: chemokine]]
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[[Category: Fernandez EJ]]
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[[Category: human stromal cell-derived factor-1alpha]]
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[[Category: Lolis E]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:48:14 2007''
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SDF-1ALPHA

PDB ID 1a15

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