6x6o
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of T4 protein Spackle as determined by native SAD phasing== | |
| + | <StructureSection load='6x6o' size='340' side='right'caption='[[6x6o]], [[Resolution|resolution]] 1.52Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X6O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x6o OCA], [https://pdbe.org/6x6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x6o RCSB], [https://www.ebi.ac.uk/pdbsum/6x6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x6o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of a bacteriophage T4 early gene product, Spackle, was determined by native sulfur single-wavelength anomalous diffraction (SAD) phasing using synchrotron radiation and was refined to 1.52 A resolution. The structure shows that Spackle consists of a bundle of five alpha-helices, forming a relatively flat disc-like overall shape. Although Spackle forms a dimer in the crystal, size-exclusion chromatography with multi-angle light scattering shows that it is monomeric in solution. Mass spectrometry confirms that purified mature Spackle lacks the amino-terminal signal peptide and contains an intramolecular disulfide bond, consistent with its proposed role in the periplasm of T4 phage-infected Escherichia coli cells. The surface electrostatic potential of Spackle shows a strikingly bipolar charge distribution, suggesting a possible mode of membrane association and inhibition of the tail lysozyme activity in T4 bacteriophage superinfection exclusion. | ||
| - | + | Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing.,Shi K, Kurniawan F, Banerjee S, Moeller NH, Aihara H Acta Crystallogr D Struct Biol. 2020 Sep 1;76(Pt 9):899-904. doi:, 10.1107/S2059798320010979. Epub 2020 Aug 25. PMID:32876065<ref>PMID:32876065</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6x6o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aihara H]] | ||
| + | [[Category: Banerjee S]] | ||
| + | [[Category: Kurniawan F]] | ||
| + | [[Category: Moeller NH]] | ||
| + | [[Category: Shi K]] | ||
Current revision
Crystal structure of T4 protein Spackle as determined by native SAD phasing
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