6wnc
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==Structure of the Rieske non-heme iron oxygenase GxtA== | ==Structure of the Rieske non-heme iron oxygenase GxtA== | ||
| - | <StructureSection load='6wnc' size='340' side='right'caption='[[6wnc]]' scene=''> | + | <StructureSection load='6wnc' size='340' side='right'caption='[[6wnc]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WNC OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6wnc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Microseira_wollei Microseira wollei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WNC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wnc OCA], [https://pdbe.org/6wnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wnc RCSB], [https://www.ebi.ac.uk/pdbsum/6wnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wnc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C3RVP5_9CYAN C3RVP5_9CYAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Biocatalysts that perform C-H hydroxylation exhibit exceptional substrate specificity and site-selectivity, often through the use of high valent oxidants to activate these inert bonds. Rieske oxygenases are examples of enzymes with the ability to perform precise mono- or dioxygenation reactions on a variety of substrates. Understanding the structural features of Rieske oxygenases responsible for control over selectivity is essential to enable the development of this class of enzymes for biocatalytic applications. Decades of research has illuminated the critical features common to Rieske oxygenases, however, structural information for enzymes that functionalize diverse scaffolds is limited. Here, we report the structures of two Rieske monooxygenases involved in the biosynthesis of paralytic shellfish toxins (PSTs), SxtT and GxtA, adding to the short list of structurally characterized Rieske oxygenases. Based on these structures, substrate-bound structures, and mutagenesis experiments, we implicate specific residues in substrate positioning and the divergent reaction selectivity observed in these two enzymes. | ||
| + | |||
| + | Structural basis for divergent C-H hydroxylation selectivity in two Rieske oxygenases.,Lukowski AL, Liu J, Bridwell-Rabb J, Narayan ARH Nat Commun. 2020 Jun 12;11(1):2991. doi: 10.1038/s41467-020-16729-0. PMID:32532989<ref>PMID:32532989</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6wnc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Microseira wollei]] | ||
[[Category: Bridwell-Rabb J]] | [[Category: Bridwell-Rabb J]] | ||
[[Category: Liu J]] | [[Category: Liu J]] | ||
Current revision
Structure of the Rieske non-heme iron oxygenase GxtA
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