6uyd

<table><tr><td colspan='2'>[[6uyd]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Hcv Hcv] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UYD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UYD FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uyd OCA], [http://pdbe.org/6uyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uyd RCSB], [http://www.ebi.ac.uk/pdbsum/6uyd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uyd ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are responsible for cell entry, with E2 being the major target of neutralizing antibodies (NAbs). Here, we present a comprehensive strategy for B cell-based HCV vaccine development through E2 optimization and nanoparticle display. We redesigned variable region 2 in a truncated form (tVR2) on E2 cores derived from genotypes 1a and 6a, resulting in improved stability and antigenicity. Crystal structures of three optimized E2 cores with human cross-genotype NAbs (AR3s) revealed how the modified tVR2 stabilizes E2 without altering key neutralizing epitopes. We then displayed these E2 cores on 24- and 60-meric nanoparticles and achieved substantial yield and purity, as well as enhanced antigenicity. In mice, these nanoparticles elicited more effective NAb responses than soluble E2 cores. Next-generation sequencing (NGS) defined distinct B cell patterns associated with nanoparticle-induced antibody responses, which target the conserved neutralizing epitopes on E2 and cross-neutralize HCV genotypes.

 

== References ==

[[Category: Hcv]]

[[Category: Human]]

[[Category: Tzarum, N]]

[[Category: Wilson, I A]]

[[Category: Zhu, J]]

[[Category: Viral protein-immune system complex]]