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| | <StructureSection load='2v9t' size='340' side='right'caption='[[2v9t]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='2v9t' size='340' side='right'caption='[[2v9t]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v9t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2V9T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v9t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V9T FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2v70|2v70]], [[2v9q|2v9q]], [[2v9r|2v9r]], [[2v9s|2v9s]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2v9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9t OCA], [http://pdbe.org/2v9t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v9t RCSB], [http://www.ebi.ac.uk/pdbsum/2v9t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9t ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9t OCA], [https://pdbe.org/2v9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v9t RCSB], [https://www.ebi.ac.uk/pdbsum/2v9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9t ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/2v9t_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/2v9t_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cusack, S]] | + | [[Category: Cusack S]] |
| - | [[Category: McCarthy, A A]] | + | [[Category: McCarthy AA]] |
| - | [[Category: Morlot, C]] | + | [[Category: Morlot C]] |
| - | [[Category: Chemotaxis]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Developmental protein]]
| + | |
| - | [[Category: Differentiation structural protein/receptor]]
| + | |
| - | [[Category: Disease mutation]]
| + | |
| - | [[Category: Egf-like domain]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Ig domain]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Ligand]]
| + | |
| - | [[Category: Lrr domain]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Neurogenesis]]
| + | |
| - | [[Category: Neuronal development]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Proto-oncogene]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Roundabout]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Slit2]]
| + | |
| - | [[Category: Structural protein-receptor complex]]
| + | |
| - | [[Category: Transmembrane]]
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| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Slits are large multidomain leucine-rich repeat (LRR)-containing proteins that provide crucial guidance cues in neuronal and vascular development. More recently, Slits have been implicated in heart morphogenesis, angiogenesis, and tumor metastasis. Slits are ligands for the Robo (Roundabout) receptors, which belong to the Ig superfamily of transmembrane signaling molecules. The Slit-Robo interaction is mediated by the second LRR domain of Slit and the two N-terminal Ig domains of Robo, but the molecular details of this interaction and how it induces signaling remain unclear. Here we describe the crystal structures of the second LRR domain of human Slit2 (Slit2 D2), the first two Ig domains of its receptor Robo1 (Ig1-2), and the minimal complex between these proteins (Slit2 D2-Robo1 Ig1). Slit2 D2 binds with its concave surface to the side of Ig1 with electrostatic and hydrophobic contact regions mediated by residues that are conserved in other family members. Surface plasmon resonance experiments and a mutational analysis of the interface confirm that Ig1 is the primary domain for binding Slit2. These structures provide molecular insight into Slit-Robo complex formation and will be important for the development of novel cancer therapeutics.
Structural insights into the Slit-Robo complex.,Morlot C, Thielens NM, Ravelli RB, Hemrika W, Romijn RA, Gros P, Cusack S, McCarthy AA Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14923-8. Epub 2007 Sep 11. PMID:17848514[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morlot C, Thielens NM, Ravelli RB, Hemrika W, Romijn RA, Gros P, Cusack S, McCarthy AA. Structural insights into the Slit-Robo complex. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14923-8. Epub 2007 Sep 11. PMID:17848514
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