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| | ==The structure of chaperone SecB in complex with unstructured proPhoA== | | ==The structure of chaperone SecB in complex with unstructured proPhoA== |
| - | <StructureSection load='5jtl' size='340' side='right'caption='[[5jtl]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5jtl' size='340' side='right'caption='[[5jtl]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jtl]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JTL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jtl]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JTL FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jtm|5jtm]], [[5jtn|5jtn]], [[5jtq|5jtq]], [[5jto|5jto]], [[5jtp|5jtp]], [[5jtr|5jtr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">secB, Z5036, ECs4487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), phoA, b0383, JW0374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtl OCA], [https://pdbe.org/5jtl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jtl RCSB], [https://www.ebi.ac.uk/pdbsum/5jtl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtl ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jtl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jtl OCA], [http://pdbe.org/5jtl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jtl RCSB], [http://www.ebi.ac.uk/pdbsum/5jtl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jtl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SECB_ECO57 SECB_ECO57]] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. | + | [https://www.uniprot.org/uniprot/SECB_ECOLI SECB_ECOLI] One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.[HAMAP-Rule:MF_00821] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Molecular chaperones act on non-native proteins in the cell to prevent their aggregation, premature folding or misfolding. Different chaperones often exert distinct effects, such as acceleration or delay of folding, on client proteins via mechanisms that are poorly understood. Here we report the solution structure of SecB, a chaperone that exhibits strong antifolding activity, in complex with alkaline phosphatase and maltose-binding protein captured in their unfolded states. SecB uses long hydrophobic grooves that run around its disk-like shape to recognize and bind to multiple hydrophobic segments across the length of non-native proteins. The multivalent binding mode results in proteins wrapping around SecB. This unique complex architecture alters the kinetics of protein binding to SecB and confers strong antifolding activity on the chaperone. The data show how the different architectures of chaperones result in distinct binding modes with non-native proteins that ultimately define the activity of the chaperone.
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| - | Structural basis for the antifolding activity of a molecular chaperone.,Huang C, Rossi P, Saio T, Kalodimos CG Nature. 2016 Aug 8. doi: 10.1038/nature18965. PMID:27501151<ref>PMID:27501151</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5jtl" style="background-color:#fffaf0;"></div>
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| | ==See Also== | | ==See Also== |
| | *[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]] | | *[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Alkaline phosphatase]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Eco57]] | + | [[Category: Escherichia coli O157:H7]] |
| - | [[Category: Ecoli]]
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Huang, C]] | + | [[Category: Huang C]] |
| - | [[Category: Kalodimos, C G]] | + | [[Category: Kalodimos CG]] |
| - | [[Category: Rossi, P]] | + | [[Category: Rossi P]] |
| - | [[Category: Saio, T]] | + | [[Category: Saio T]] |
| - | [[Category: Chaperone-hydrolase complex]]
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| - | [[Category: Molecular chaperone]]
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