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1c0m

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(New page: 200px<br /> <applet load="1c0m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c0m, resolution 2.53&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1c0m.gif|left|200px]]<br />
 
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<applet load="1c0m" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1c0m, resolution 2.53&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RSV TWO-DOMAIN INTEGRASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RSV TWO-DOMAIN INTEGRASE==
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Integration of retroviral cDNA is a necessary step in viral replication., The virally encoded integrase protein and DNA sequences at the ends of the, linear viral cDNA are required for this reaction. Previous studies, revealed that truncated forms of Rous sarcoma virus integrase containing, two of the three protein domains can carry out integration reactions in, vitro. Here, we describe the crystal structure at 2.5 A resolution of a, fragment of the integrase of Rous sarcoma virus (residues 49-286), containing both the conserved catalytic domain and a modulatory, DNA-binding domain (C domain). The catalytic domains form a symmetric, dimer, but the C domains associate asymmetrically with each other and, together adopt a canted conformation relative to the catalytic domains. A, binding path for the viral cDNA is evident spanning both domain surfaces, allowing modeling of the larger integration complexes that are known to be, active in vivo. The modeling suggests that formation of an integrase, tetramer (a dimer of dimers) is necessary and sufficient for joining both, viral cDNA ends at neighboring sites in the target DNA. The observed, asymmetric arrangement of C domains suggests that they could form a, rotationally symmetric tetramer that may be important for bridging, integrase complexes at each cDNA end.
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<StructureSection load='1c0m' size='340' side='right'caption='[[1c0m]], [[Resolution|resolution]] 2.53&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1c0m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C0M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.53&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c0m OCA], [https://pdbe.org/1c0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c0m RCSB], [https://www.ebi.ac.uk/pdbsum/1c0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c0m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POL_RSVP POL_RSVP] Capsid protein p27 forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/1c0m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c0m ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1C0M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C0M OCA].
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of an active two-domain derivative of Rous sarcoma virus integrase., Yang ZN, Mueser TC, Bushman FD, Hyde CC, J Mol Biol. 2000 Feb 18;296(2):535-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10669607 10669607]
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[[Category: Large Structures]]
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[[Category: RNA-directed DNA polymerase]]
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[[Category: Rous sarcoma virus]]
[[Category: Rous sarcoma virus]]
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[[Category: Single protein]]
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[[Category: Bushman FD]]
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[[Category: Bushman, F.D.]]
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[[Category: Hyde CC]]
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[[Category: Hyde, C.C.]]
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[[Category: Mueser TC]]
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[[Category: Mueser, T.C.]]
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[[Category: Yang Z-N]]
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[[Category: Yang, Z.N.]]
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[[Category: hiv]]
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[[Category: integrase]]
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[[Category: protein structure]]
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[[Category: rous sarcoma virus]]
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[[Category: transferase]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:55:24 2007''
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Current revision

CRYSTAL STRUCTURE OF RSV TWO-DOMAIN INTEGRASE

PDB ID 1c0m

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