This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1eia

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1eia" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eia, resolution 2.7&Aring;" /> '''X-RAY CRYSTAL STRUCT...)
Current revision (10:02, 20 March 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1eia.gif|left|200px]]<br />
 
-
<applet load="1eia" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1eia, resolution 2.7&Aring;" />
 
-
'''X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26'''<br />
 
-
==Overview==
+
==X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26==
-
Two crystal forms of recombinant p26 capsid protein (CA) from the equine, infectious anemia virus (EIAV) have in common an antiparallel four-helix, bundle dimer interface between N-terminal domains (NTDs). The dimer, interface provides a lenient scaffold to accommodate the wide sequence, variation in these helices within lentivirus CA. Pairs of dimers weakly, associate to form exact or approximate D2 symmetry tetramers. In one of, the two crystal forms, the tetramers are linked via dimerization of, C-terminal domains (CTDs). We propose that the observed NTD and CTD, homodimer interactions are involved in the assembly of the lentivirus, capsid. The NTD homodimer shape readily suggests a model for the mature, capsid core, based on hexagonal packing with dimensions and surface, topology resembling described EIAV capsid cores. Combining available data, for human immunodeficiency virus and EIAV CA, we also propose an assembly, pathway for maturation of the lentivirus capsid core following proteolytic, cleavage of the gag polyprotein precursor.
+
<StructureSection load='1eia' size='340' side='right'caption='[[1eia]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1eia]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIA FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eia OCA], [https://pdbe.org/1eia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eia RCSB], [https://www.ebi.ac.uk/pdbsum/1eia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eia ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GAG_EIAVY GAG_EIAVY] Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane (By similarity). Capsid protein p26 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p11 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity). p9 plays a role in budding of the assembled particle by interacting with PDCD6IP/AIP1 (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eia_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eia ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1EIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EIA OCA].
+
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Model for lentivirus capsid core assembly based on crystal dimers of EIAV p26., Jin Z, Jin L, Peterson DL, Lawson CL, J Mol Biol. 1999 Feb 12;286(1):83-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9931251 9931251]
+
[[Category: Equine infectious anemia virus]]
[[Category: Equine infectious anemia virus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Jin, L.]]
+
[[Category: Jin L]]
-
[[Category: Jin, Z.]]
+
[[Category: Jin Z]]
-
[[Category: Lawson, C.L.]]
+
[[Category: Lawson CL]]
-
[[Category: Peterson, D.L.]]
+
[[Category: Peterson DL]]
-
[[Category: crystal structure]]
+
-
[[Category: eiav]]
+
-
[[Category: hiv]]
+
-
[[Category: lentivirus]]
+
-
[[Category: viral capsid]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:00:56 2007''
+

Current revision

X-RAY CRYSTAL STRUCTURE OF EQUINE INFECTIOUS ANEMIA VIRUS (EIAV) CAPSID PROTEIN P26

PDB ID 1eia

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1eia"
Personal tools