6x44
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==High Resolution Crystal Structure Analysis of SERA5 proenzyme from plasmodium falciparum== | |
| + | <StructureSection load='6x44' size='340' side='right'caption='[[6x44]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6x44]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X44 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X44 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1973395Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x44 OCA], [https://pdbe.org/6x44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x44 RCSB], [https://www.ebi.ac.uk/pdbsum/6x44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x44 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SERA5_PLAF7 SERA5_PLAF7] Plays an essential role during the asexual blood stage development by controlling the kinetics of merozoite egress from host erythrocytes (PubMed:25599609, PubMed:28683142). Specifically, prevents premature rupture of the parasitophorous vacuole and host erythrocyte membranes (PubMed:28683142).<ref>PMID:25599609</ref> <ref>PMID:28683142</ref> May prevent merozoite phagocytosis by host monocytes via interaction with host VTN at the merozoite surface (By similarity). Plays a role in parasite growth (By similarity).[UniProtKB:P69193] Protease activity is controversial (PubMed:25599609). Has been shown in a number of studies to have protease activity towards a synthetic peptide in vitro (PubMed:13679369, PubMed:24769454, PubMed:29716996). Has also been shown to lack protease activity towards a synthetic peptide in vitro (PubMed:25599609).<ref>PMID:13679369</ref> <ref>PMID:24769454</ref> <ref>PMID:25599609</ref> <ref>PMID:29716996</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PfSERA5, a significantly abundant protein present within the parasitophorous vacuole (PV) and essential for normal growth during the blood-stage life cycle of the malaria parasite Plasmodium falciparum, displays structural similarity to many other cysteine proteases. However, PfSERA5 does not exhibit any detectable protease activity and therefore the role of the PfSERA5 papain-like domain (PfSERA5E), thought to remain bound to its cognate prodomain, remains unknown. In this study, we present a revised structure of the central PfSERA5E domain at a resolution of 1.2 A, and the first structure of the "zymogen" of this papain-like domain including its cognate prodomain (PfSERA5PE) to 2.2 A resolution. PfSERA5PE is somewhat structurally similar to that of other known proenzymes, retaining the conserved overall folding and orientation of the prodomain through, and occluding, the archetypal papain-like catalytic triad "active-site" cleft, in the same reverse direction as conventional prodomains. Our findings are congruent with previously identified structures of PfSERA5E and of similar "zymogens" and provide a foundation for further investigation into the function of PfSERA5. | ||
| - | + | Structure of the Plasmodium falciparum PfSERA5 pseudo-zymogen.,Smith NA, Clarke OB, Lee M, Hodder AN, Smith BJ Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3956. PMID:32955133<ref>PMID:32955133</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6x44" style="background-color:#fffaf0;"></div> |
| - | [[Category: Clarke | + | == References == |
| - | [[Category: Lee | + | <references/> |
| - | [[Category: Smith | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Plasmodium falciparum]] | ||
| + | [[Category: Clarke OB]] | ||
| + | [[Category: Lee M]] | ||
| + | [[Category: Smith BJ]] | ||
| + | [[Category: Smith NA]] | ||
Current revision
High Resolution Crystal Structure Analysis of SERA5 proenzyme from plasmodium falciparum
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