1bvo

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[[Image:1bvo.gif|left|200px]]
 
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==DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA==
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The line below this paragraph, containing "STRUCTURE_1bvo", creates the "Structure Box" on the page.
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<StructureSection load='1bvo' size='340' side='right'caption='[[1bvo]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1bvo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BVO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bvo OCA], [https://pdbe.org/1bvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bvo RCSB], [https://www.ebi.ac.uk/pdbsum/1bvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bvo ProSAT]</span></td></tr>
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{{STRUCTURE_1bvo| PDB=1bvo | SCENE= }}
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</table>
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== Function ==
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'''DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA'''
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[https://www.uniprot.org/uniprot/Q17034_ANOGA Q17034_ANOGA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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BACKGROUND: NF-kappa B/Rel transcription factors play important roles in immunity and development in mammals and insects. Their activity is regulated by their cellular localization, homo- and heterodimerization and association with other factors on their target gene promoters. Gambif1 from Anopheles gambiae is a member of the Rel family and a close homologue of the morphogen Dorsal, which establishes dorsoventral polarity in the Drosophila embryo. RESULTS: We present the crystal structure of the N-terminal specificity domain of Gambif1 bound to DNA. This first structure of an insect Rel protein-DNA complex shows that Gambif1 binds a GGG half-site element using a stack of three arginine sidechains. Differences in affinity to Dorsal binding sites in target gene promoters are predicted to arise from base changes in these GGG elements. An arginine that is conserved in class II Rel proteins (members of which contain a transcription activation domain) contacts the outermost guanines of the DNA site. This previously unseen specific contact contributes strongly to the DNA-binding affinity and might be responsible for differences in specificity between Rel proteins of class I and II. CONCLUSIONS: The Gambif1-DNA complex structure illustrates how differences in Dorsal affinity to binding sites in developmental gene promoters are achieved. Comparison with other Rel-DNA complex structures leads to a general model for DNA recognition by Rel proteins.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bv/1bvo_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1BVO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVO OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bvo ConSurf].
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Structure of the specificity domain of the Dorsal homologue Gambif1 bound to DNA., Cramer P, Varrot A, Barillas-Mury C, Kafatos FC, Muller CW, Structure. 1999 Jul 15;7(7):841-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10425685 10425685]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Anopheles gambiae]]
[[Category: Anopheles gambiae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barillas-Mury, C.]]
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[[Category: Barillas-Mury C]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Kafatos, F C.]]
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[[Category: Kafatos FC]]
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[[Category: Mueller, C W.]]
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[[Category: Mueller CW]]
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[[Category: Varrot, A.]]
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[[Category: Varrot A]]
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[[Category: Development]]
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[[Category: Immunity]]
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[[Category: Insect]]
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[[Category: Morphogen]]
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[[Category: Rel protein]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:00:35 2008''
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Current revision

DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA

PDB ID 1bvo

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