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1ggb

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(New page: 200px<br /> <applet load="1ggb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ggb, resolution 2.8&Aring;" /> '''MAJOR ANTIGEN-INDUCE...)
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[[Image:1ggb.gif|left|200px]]<br />
 
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<applet load="1ggb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ggb, resolution 2.8&Aring;" />
 
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'''MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY'''<br />
 
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==Overview==
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==MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY==
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BACKGROUND: Recent structural results have shown that antibodies use an, induced fit mechanism to recognize and bind their antigens. Here we, present the crystallographically determined structure of an Fab directed, against an HIV-1 peptide (Fab 50.1) in the unliganded state and compare it, with the peptide-bound structure. We perform a detailed analysis of the, components that contribute to enhanced antigen binding and recognition., RESULTS: Induced fit of Fab 50.1 to its peptide antigen involves a, substantial rearrangement of the third complementarity determining region, loop of the heavy chain (H3), as well as a large rotation of the variable, heavy (VH) chain relative to the variable light (VL) chain. Analysis of, other Fab structures suggests that the extent of the surface area buried, at the VL-VH interface correlates with the ability to alter antibody, quaternary structure by reorientation of the VL-VH domains. CONCLUSION:, Fab 50.1 exhibits the largest conformational changes yet observed in a, single antibody. These can be attributed to the flexibility of the, variable region. Comparisons of new data with previous examples lend to, the general conclusion that a small VL-VH interface, due in part to a, short H3 loop, permits substantial alterations to the antigen-binding, pocket. This has major implications for the prediction, engineering and, design of antibody-combining sites.
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<StructureSection load='1ggb' size='340' side='right'caption='[[1ggb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ggb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GGB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ggb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ggb OCA], [https://pdbe.org/1ggb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ggb RCSB], [https://www.ebi.ac.uk/pdbsum/1ggb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ggb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCAM_MOUSE GCAM_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gg/1ggb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ggb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GGB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GGB OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Major antigen-induced domain rearrangements in an antibody., Stanfield RL, Takimoto-Kamimura M, Rini JM, Profy AT, Wilson IA, Structure. 1993 Oct 15;1(2):83-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8069628 8069628]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Takimoto-Kamimura, M.]]
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[[Category: Large Structures]]
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[[Category: Wilson, I.A.]]
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[[Category: Mus musculus]]
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[[Category: immunoglobulin]]
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[[Category: Takimoto-Kamimura M]]
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[[Category: Wilson IA]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:04:22 2007''
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Current revision

MAJOR ANTIGEN-INDUCED DOMAIN REARRANGEMENTS IN AN ANTIBODY

PDB ID 1ggb

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