|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution Structure of alpha-Conotoxin OmIA== | | ==Solution Structure of alpha-Conotoxin OmIA== |
| - | <StructureSection load='2gcz' size='340' side='right'caption='[[2gcz]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2gcz' size='340' side='right'caption='[[2gcz]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2gcz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2GCZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2gcz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_omaria Conus omaria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GCZ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2gcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcz OCA], [http://pdbe.org/2gcz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gcz RCSB], [http://www.ebi.ac.uk/pdbsum/2gcz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gcz ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcz OCA], [https://pdbe.org/2gcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gcz RCSB], [https://www.ebi.ac.uk/pdbsum/2gcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gcz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CA1A_CONOM CA1A_CONOM]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks alpha-3/beta-2 (IC(50)=11 nM), alpha-7 (IC(50)=27.1) and alpha-6/alpha-3-beta-2-beta-3 (IC(50)=201 nM) subunits of nAChR.<ref>PMID:16803900</ref> | + | [https://www.uniprot.org/uniprot/CA1A_CONOM CA1A_CONOM] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks alpha-3/beta-2 (IC(50)=11 nM), alpha-7 (IC(50)=27.1) and alpha-6/alpha-3-beta-2-beta-3 (IC(50)=201 nM) subunits of nAChR.<ref>PMID:16803900</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Conus omaria]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chi, S W]] | + | [[Category: Chi S-W]] |
| - | [[Category: Han, K H]] | + | [[Category: Han K-H]] |
| - | [[Category: Kim, D H]] | + | [[Category: Kim D-H]] |
| - | [[Category: McIntosh, J M]] | + | [[Category: McIntosh JM]] |
| - | [[Category: Olivera, B M]] | + | [[Category: Olivera BM]] |
| - | [[Category: Alpha-helix]]
| + | |
| - | [[Category: Beta-turn]]
| + | |
| - | [[Category: C-terminal amidation]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Two disulfide bond]]
| + | |
| Structural highlights
Function
CA1A_CONOM Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks alpha-3/beta-2 (IC(50)=11 nM), alpha-7 (IC(50)=27.1) and alpha-6/alpha-3-beta-2-beta-3 (IC(50)=201 nM) subunits of nAChR.[1]
Publication Abstract from PubMed
alpha-Conotoxin OmIA from Conus omaria is the only alpha-conotoxin that shows a approximately 20-fold higher affinity to the alpha3beta2 over the alpha6beta2 subtype of nicotinic acetylcholine receptor. We have determined a three-dimensional structure of alpha-conotoxin OmIA by nuclear magnetic resonance spectroscopy. alpha-Conotoxin OmIA has an "omega-shaped" overall topology with His(5)-Asn(12) forming an alpha-helix. Structural features of alpha-conotoxin OmIA responsible for its selectivity are suggested by comparing its surface characteristics with other functionally related alpha4/7 subfamily conotoxins. Reduced size of the hydrophilic area in alpha-conotoxin OmIA seems to be associated with the reduced affinity towards the alpha6beta2 nAChR subtype.
Solution conformation of a neuronal nicotinic acetylcholine receptor antagonist alpha-conotoxin OmIA that discriminates alpha3 vs. alpha6 nAChR subtypes.,Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH Biochem Biophys Res Commun. 2006 Jun 23;345(1):248-54. Epub 2006 Apr 27. PMID:16678128[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Talley TT, Olivera BM, Han KH, Christensen SB, Dowell C, Tsigelny I, Ho KY, Taylor P, McIntosh JM. Alpha-conotoxin OmIA is a potent ligand for the acetylcholine-binding protein as well as alpha3beta2 and alpha7 nicotinic acetylcholine receptors. J Biol Chem. 2006 Aug 25;281(34):24678-86. doi: 10.1074/jbc.M602969200. Epub 2006, Jun 27. PMID:16803900 doi:http://dx.doi.org/10.1074/jbc.M602969200
- ↑ Chi SW, Kim DH, Olivera BM, McIntosh JM, Han KH. Solution conformation of a neuronal nicotinic acetylcholine receptor antagonist alpha-conotoxin OmIA that discriminates alpha3 vs. alpha6 nAChR subtypes. Biochem Biophys Res Commun. 2006 Jun 23;345(1):248-54. Epub 2006 Apr 27. PMID:16678128 doi:http://dx.doi.org/S0006-291X(06)00889-8
|
