2jak

From Proteopedia

(Difference between revisions)

Current revision

Human PP2A regulatory subunit B56g

Structural highlights

2jak is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

2A5G_HUMAN The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The PP2A serine/threonine phosphatase regulates a plethora of cellular processes. In the cell the predominant form of the enzyme is a heterotrimer, formed by a core dimer composed of a catalytic and a scaffolding subunit, which assemble together with one of a range of different regulatory B subunits. Here, we present the first structure of a free non-complexed B subunit, B56 gamma. Comparison with the recent structures of a heterotrimeric complex and the core dimer reveals several significant conformational changes in the interface region between the B56 gamma and the core dimer. These allow for an assembly scheme of the PP2A holoenzyme to be put forth where B56 gamma first complexes with the scaffolding subunit and subsequently binds to the catalytic subunit and this induces the formation of a binding site for the invariant C-terminus of the catalytic subunit that locks in the complex as a last step of assembly.

The structure of the PP2A regulatory subunit B56 gamma: the remaining piece of the PP2A jigsaw puzzle.,Magnusdottir A, Stenmark P, Flodin S, Nyman T, Kotenyova T, Graslund S, Ogg D, Nordlund P Proteins. 2009 Jan;74(1):212-21. PMID:18618707^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Letourneux C, Rocher G, Porteu F. B56-containing PP2A dephosphorylate ERK and their activity is controlled by the early gene IEX-1 and ERK. EMBO J. 2006 Feb 22;25(4):727-38. Epub 2006 Feb 2. PMID:16456541 doi:http://dx.doi.org/10.1038/sj.emboj.7600980
↑ Li HH, Cai X, Shouse GP, Piluso LG, Liu X. A specific PP2A regulatory subunit, B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55. EMBO J. 2007 Jan 24;26(2):402-11. PMID:17245430 doi:http://dx.doi.org/10.1038/sj.emboj.7601519
↑ Magnusdottir A, Stenmark P, Flodin S, Nyman T, Kotenyova T, Graslund S, Ogg D, Nordlund P. The structure of the PP2A regulatory subunit B56 gamma: the remaining piece of the PP2A jigsaw puzzle. Proteins. 2009 Jan;74(1):212-21. PMID:18618707 doi:10.1002/prot.22150

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jak"

@@ Line 3: / Line 3: @@
 <StructureSection load='2jak' size='340' side='right'caption='[[2jak]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JAK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2JAK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JAK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2jak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jak OCA], [http://pdbe.org/2jak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jak RCSB], [http://www.ebi.ac.uk/pdbsum/2jak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jak ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jak OCA], [https://pdbe.org/2jak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jak RCSB], [https://www.ebi.ac.uk/pdbsum/2jak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jak ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/2A5G_HUMAN 2A5G_HUMAN] The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.<ref>PMID:16456541</ref> <ref>PMID:17245430</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Arrowsmith, C]]
+[[Category: Arrowsmith C]]
-[[Category: Berg, S Van Den]]
+[[Category: Berglund H]]
-[[Category: Berglund, H]]
+[[Category: Busam R]]
-[[Category: Busam, R]]
+[[Category: Collins R]]
-[[Category: Collins, R]]
+[[Category: Edwards A]]
-[[Category: Edwards, A]]
+[[Category: Ericsson UB]]
-[[Category: Ericsson, U B]]
+[[Category: Flodin S]]
-[[Category: Flodin, S]]
+[[Category: Flores A]]
-[[Category: Flores, A]]
+[[Category: Graslund S]]
-[[Category: Graslund, S]]
+[[Category: Hallberg BM]]
-[[Category: Hallberg, B M]]
+[[Category: Hammarstrom M]]
-[[Category: Hammarstrom, M]]
+[[Category: Hogbom M]]
-[[Category: Hogbom, M]]
+[[Category: Holmberg Schiavone L]]
-[[Category: Johansson, I]]
+[[Category: Johansson I]]
-[[Category: Karlberg, T]]
+[[Category: Karlberg T]]
-[[Category: Kotenyova, T]]
+[[Category: Kotenyova T]]
-[[Category: Lundgren, S]]
+[[Category: Lundgren S]]
-[[Category: Magnusdottir, A]]
+[[Category: Magnusdottir A]]
-[[Category: Moche, M]]
+[[Category: Moche M]]
-[[Category: Nilsson, P]]
+[[Category: Nilsson P]]
-[[Category: Nordlund, P]]
+[[Category: Nordlund P]]
-[[Category: Nyman, T]]
+[[Category: Nyman T]]
-[[Category: Ogg, D]]
+[[Category: Ogg D]]
-[[Category: Persson, C]]
+[[Category: Persson C]]
-[[Category: Sagemark, J]]
+[[Category: Sagemark J]]
-[[Category: Schiavone, L Holmberg]]
+[[Category: Stenmark P]]
-[[Category: Stenmark, P]]
+[[Category: Sundstrom M]]
-[[Category: Sundstrom, M]]
+[[Category: Thorsell AG]]
-[[Category: Thorsell, A G]]
+[[Category: Uppenberg J]]
-[[Category: Uppenberg, J]]
+[[Category: Upsten M]]
-[[Category: Upsten, M]]
+[[Category: Van Den Berg S]]
-[[Category: Weigelt, J]]
+[[Category: Weigelt J]]
-[[Category: B56g]]
-[[Category: Nuclear protein]]
-[[Category: Phosphorylation]]
-[[Category: Pp2a]]
-[[Category: Pp2a regulatory subunit]]
-[[Category: Ppp2r5c]]

2jak

From Proteopedia

Current revision

Human PP2A regulatory subunit B56g

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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