|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='2vnv' size='340' side='right'caption='[[2vnv]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='2vnv' size='340' side='right'caption='[[2vnv]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vnv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcj Burcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VNV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VNV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vnv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_J2315 Burkholderia cenocepacia J2315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VNV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vnv OCA], [http://pdbe.org/2vnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vnv RCSB], [http://www.ebi.ac.uk/pdbsum/2vnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vnv ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vnv OCA], [https://pdbe.org/2vnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vnv RCSB], [https://www.ebi.ac.uk/pdbsum/2vnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vnv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B4EH87_BURCJ B4EH87_BURCJ] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 30: |
Line 33: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Burcj]] | + | [[Category: Burkholderia cenocepacia J2315]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Imberty, A]] | + | [[Category: Imberty A]] |
| - | [[Category: Lameignere, E]] | + | [[Category: Lameignere E]] |
| - | [[Category: Malinovska, L]] | + | [[Category: Malinovska L]] |
| - | [[Category: Mitchell, E P]] | + | [[Category: Mitchell EP]] |
| - | [[Category: Wimmerova, M]] | + | [[Category: Wimmerova M]] |
| - | [[Category: Burkholderia cenocepacia]]
| + | |
| - | [[Category: Protein sugar interaction]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| - | [[Category: Sugar-binding protein]]
| + | |
| - | [[Category: Sugar-binding protein lectin]]
| + | |
| Structural highlights
Function
B4EH87_BURCJ
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Chronic colonization of the lungs by opportunist bacteria such as Pseudomonas aeruginosa and members of the Bcc (Burkholderia cepacia complex) is the major cause of morbidity and mortality among CF (cystic fibrosis) patients. PA-IIL (lecB gene), a soluble lectin from Ps. aeruginosa, has been the subject of much interest because of its very strong affinity for fucose. Orthologues have been identified in the opportunist bacteria Ralstonia solanacearum, Chromobacterium violaceum and Burkholderia of Bcc. The genome of the J2315 strain of B. cenocepacia, responsible for epidemia in CF centres, contains three genes that code for proteins with PA-IIL domains. The shortest gene was cloned in Escherichia coli and pure recombinant protein, BclA (B. cenocepacia lectin A), was obtained. The presence of native BclA in B. cenocepacia extracts was checked using a proteomic approach. The specificity of recombinant BclA was characterized using surface plasmon resonance showing a preference for mannosides and supported with glycan array experiments demonstrating a strict specificity for oligomannose-type N-glycan structures. The interaction thermodynamics of BclA with methyl alpha-D-mannoside demonstrates a dissociation constant (K(d)) of 2.75 x 10(-6) M. The X-ray crystal structure of the complex with methyl alpha-D-mannoside was determined at 1.7 A (1 A=0.1 nm) resolution. The lectin forms homodimers with one binding site per monomer, acting co-operatively with the second dimer site. Each monomer contains two Ca2+ ions and one sugar ligand. Despite strong sequence similarity, the differences between BclA and PA-IIL in their specificity, binding site and oligomerization mode indicate that the proteins should have different roles in the bacteria.
Structural basis for mannose recognition by a lectin from opportunistic bacteria Burkholderia cenocepacia.,Lameignere E, Malinovska L, Slavikova M, Duchaud E, Mitchell EP, Varrot A, Sedo O, Imberty A, Wimmerova M Biochem J. 2008 Apr 15;411(2):307-18. PMID:18215132[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lameignere E, Malinovska L, Slavikova M, Duchaud E, Mitchell EP, Varrot A, Sedo O, Imberty A, Wimmerova M. Structural basis for mannose recognition by a lectin from opportunistic bacteria Burkholderia cenocepacia. Biochem J. 2008 Apr 15;411(2):307-18. PMID:18215132 doi:10.1042/bj20071276
|
