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| | <StructureSection load='2wfk' size='340' side='right'caption='[[2wfk]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='2wfk' size='340' side='right'caption='[[2wfk]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wfk]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptospira_santarosai_serovar_shermani Leptospira santarosai serovar shermani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WFK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wfk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_santarosai_serovar_Shermani Leptospira santarosai serovar Shermani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WFK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfk OCA], [http://pdbe.org/2wfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wfk RCSB], [http://www.ebi.ac.uk/pdbsum/2wfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfk OCA], [https://pdbe.org/2wfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wfk RCSB], [https://www.ebi.ac.uk/pdbsum/2wfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6S9S1_9LEPT Q6S9S1_9LEPT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leptospira santarosai serovar shermani]] | + | [[Category: Leptospira santarosai serovar Shermani]] |
| - | [[Category: Sun, Y J]] | + | [[Category: Sun Y-J]] |
| - | [[Category: Tung, J Y]] | + | [[Category: Tung J-Y]] |
| - | [[Category: Yang, C W]] | + | [[Category: Yang C-W]] |
| - | [[Category: Ca2+-bound]]
| + | |
| - | [[Category: Calcium binding]]
| + | |
| - | [[Category: Extracellular matrix outer membrane protein]]
| + | |
| - | [[Category: Fibronectin]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Metal binding protein]]
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| Structural highlights
Function
Q6S9S1_9LEPT
Publication Abstract from PubMed
Tubulointerstitial nephritis is a cardinal renal manifestation of leptospirosis. LipL32, a major lipoprotein and a virulence factor, locates on the outer membrane of the pathogen Leptospira. It evades immune response by recognizing and adhering to extracellular matrix components of the host cell. The crystal structure of Ca(2+)-bound LipL32 was determined at 2.3 A resolution. LipL32 has a novel polyD sequence of seven aspartates that forms a continuous acidic surface patch for Ca(2+) binding. A significant conformational change was observed for the Ca(2+)-bound form of LipL32. Calcium binding to LipL32 was determined by isothermal titration calorimetry. The binding of fibronectin to LipL32 was observed by Stains-all CD and enzyme-linked immunosorbent assay experiments. The interaction between LipL32 and fibronectin might be associated with Ca(2+) binding. Based on the crystal structure of Ca(2+)-bound LipL32 and the Stains-all results, fibronectin probably binds near the polyD region on LipL32. Ca(2+) binding to LipL32 might be important for Leptospira to interact with the extracellular matrix of the host cell.
Calcium binds to LipL32, a lipoprotein from pathogenic Leptospira, and modulates fibronectin binding.,Tung JY, Yang CW, Chou SW, Lin CC, Sun YJ J Biol Chem. 2010 Jan 29;285(5):3245-52. Epub 2009 Nov 30. PMID:19948735[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tung JY, Yang CW, Chou SW, Lin CC, Sun YJ. Calcium binds to LipL32, a lipoprotein from pathogenic Leptospira, and modulates fibronectin binding. J Biol Chem. 2010 Jan 29;285(5):3245-52. Epub 2009 Nov 30. PMID:19948735 doi:10.1074/jbc.M109.006320
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