5k7t

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<StructureSection load='5k7t' size='340' side='right'caption='[[5k7t]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='5k7t' size='340' side='right'caption='[[5k7t]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5k7t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K7T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5k7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K7T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k7n|5k7n]], [[5k7o|5k7o]], [[5k7p|5k7p]], [[5k7q|5k7q]], [[5k7r|5k7r]], [[5k7s|5k7s]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7t OCA], [https://pdbe.org/5k7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k7t RCSB], [https://www.ebi.ac.uk/pdbsum/5k7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7t ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7t OCA], [http://pdbe.org/5k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k7t RCSB], [http://www.ebi.ac.uk/pdbsum/5k7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7t ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
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[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
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Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.,de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T Nat Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178. PMID:28192420<ref>PMID:28192420</ref>
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==See Also==
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*[[Thermolysin 3D structures|Thermolysin 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5k7t" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus thermoproteolyticus]]
[[Category: Bacillus thermoproteolyticus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thermolysin]]
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[[Category: Cascio D]]
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[[Category: Cascio, D]]
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[[Category: Eisenberg D]]
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[[Category: Cruz, M J.de la]]
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[[Category: Gonen T]]
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[[Category: Eisenberg, D]]
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[[Category: Hattne J]]
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[[Category: Gonen, T]]
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[[Category: Reyes FE]]
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[[Category: Hattne, J]]
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[[Category: Rodriguez J]]
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[[Category: Reyes, F E]]
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[[Category: Sawaya MR]]
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[[Category: Rodriguez, J]]
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[[Category: Seidler P]]
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[[Category: Sawaya, M R]]
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[[Category: Shi D]]
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[[Category: Seidler, P]]
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[[Category: De la Cruz MJ]]
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[[Category: Shi, D]]
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[[Category: Hydrolase]]
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Current revision

MicroED structure of thermolysin at 2.5 A resolution

PDB ID 5k7t

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