1ihv

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(New page: 200px<br /> <applet load="1ihv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ihv" /> '''SOLUTION STRUCTURE OF THE DNA BINDING DOMAI...)
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[[Image:1ihv.gif|left|200px]]<br />
 
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<applet load="1ihv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ihv" />
 
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'''SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE==
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The solution structure of the DNA binding domain of HIV-1 integrase, (residues 220-270) has been determined by multidimensional NMR, spectroscopy. The protein is a dimer in solution, and each subunit is, composed of a five-stranded beta-barrel with a topology very similar to, that of the SH3 domain. The dimer is formed by a stacked beta-interface, comprising strands 2, 3, and 4, with the two triple-stranded antiparallel, beta-sheets, one from each subunit, oriented antiparallel to each other., One surface of the dimer, bounded by the loop between strands beta 1 and, beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x, 23 x 12 A in cross section. Lys264, which has been shown from mutational, data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.
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<StructureSection load='1ihv' size='340' side='right'caption='[[1ihv]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ihv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ihv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihv OCA], [https://pdbe.org/1ihv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ihv RCSB], [https://www.ebi.ac.uk/pdbsum/1ihv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ihv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POL_HV1Z6 POL_HV1Z6] Integrase performs the integration of the newly synthesized dsDNA copy of the viral genome into the host chromosome. The integrated DNA is called provirus.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.
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==About this Structure==
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Solution structure of the DNA binding domain of HIV-1 integrase.,Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683<ref>PMID:7632683</ref>
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1IHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IHV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the DNA binding domain of HIV-1 integrase., Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM, Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7632683 7632683]
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</div>
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[[Category: Human immunodeficiency virus]]
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<div class="pdbe-citations 1ihv" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Clore, G.M.]]
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[[Category: Ernst, J.A.]]
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[[Category: Gronenborn, A.M.]]
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[[Category: Lodi, P.J.]]
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[[Category: aids]]
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[[Category: dna-binding protein]]
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[[Category: polyprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:10:00 2007''
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==See Also==
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Human immunodeficiency virus 1]]
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[[Category: Large Structures]]
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[[Category: Clore GM]]
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[[Category: Ernst JA]]
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[[Category: Gronenborn AM]]
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[[Category: Lodi PJ]]

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SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE

PDB ID 1ihv

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